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5 hits found for Relaxase

SASDX82 – ...relaxase from Bacillus subtilis conjugative plasmid pLS20

Relaxase experimental SAS data
PHENIX model
Sample: Relaxase monomer, 29 kDa Bacillus subtilis subsp. … protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
...relaxase from Bacillus subtilis conjugative plasmid pLS20 reveals a new mechanism of DNA processing mediated by dimerization
Isidro Crespo
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 48 nm3

SASDX92 – ...relaxase from Bacillus subtilis conjugative plasmid pLS20 in complex with nic sequence DNA

Relaxasenic sequence, single stranded experimental SAS data
PHENIX model
Sample: Relaxase dimer, 57 kDa Bacillus subtilis subsp. … protein
nic sequence, single stranded dimer, 12 kDa Bacillus subtilis DNA
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
...relaxase from Bacillus subtilis conjugative plasmid pLS20 reveals a new mechanism of DNA processing mediated by dimerization
Isidro Crespo
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDED9Relaxase domain of TraI

Relaxase (Tra_2) domain of TraI experimental SAS data
GASBOR model
Sample: Relaxase (Tra_2) domain of TraI monomer, 46 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
...relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 61 nm3

SASDEE9 – TraI_2_C domain of TraI

TraI_2_C domain of TraI experimental SAS data
GASBOR model
Sample: TraI_2_C domain of TraI monomer, 21 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
...relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDEF9 – TraI of Neisseria gonorrhoeae

TraI experimental SAS data
GASBOR model
Sample: TraI monomer, 91 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 5
...relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 7.3 nm
Dmax 31.4 nm
VolumePorod 293 nm3