Small Angle Scattering Biological Data Bank SASBDB


Advanced search

5 hits found for Replicase polyprotein 1a - nsp7

SASDP23 – ...nsp7-11) polyprotein monomer

Replicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E

R_g^Guinier	3.5	nm
D_max	15.6	nm
Volume^Porod	102	nm³

SASDP33 – ...nsp7-11) polyprotein dimer

Sample:	Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E

R_g^Guinier	4.6	nm
D_max	19.1	nm
Volume^Porod	203	nm³

SASDKJ4 – ...nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data

Sample:	Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	92	nm³

SASDMJ9 – ...Nsp7-10 polyprotein

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R

R_g^Guinier

2.1

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2