Small Angle Scattering Biological Data Bank SASBDB


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146 hits found for Lucas

SASDGX8 – Truncated Apolipoprotein E4 (1-191) bound to 110 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data

Apolipoprotein E4 (1-191) Suramin Kratky plot

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.8	nm
D_max	9.5	nm

SASDNX8 – Complex glycan contactin-2 immunoglobulin domains1-6, 5μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	353	nm³

SASDFY8 – Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.1	nm
D_max	24.0	nm
Volume^Porod	666	nm³

SASDGY8 – Truncated Apolipoprotein E4 (1-191) bound to 37 µM Suramin

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.6	nm
D_max	8.0	nm

SASDNY8 – Complex glycan contactin-2 immunoglobulin domains1-6, 11μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	365	nm³

SASDFZ8 – Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.2	nm
D_max	24.5	nm
Volume^Porod	696	nm³

SASDGZ8 – Truncated Apolipoprotein E4 (1-191) bound to 12 µM Suramin

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.5	nm
D_max	8.2	nm

SASDNZ8 – Complex glycan contactin-2 immunoglobulin domains1-6, 24μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.6	nm
D_max	22.0	nm
Volume^Porod	390	nm³

SASDG29 – Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.4	nm
D_max	7.8	nm

SASDN29 – Complex glycan contactin-2 immunoglobulin domains1-6, 51μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 7

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.8	nm
D_max	32.0	nm
Volume^Porod	412	nm³

SASDG39 – Apolipoprotein E2

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.5	nm

SASDN39 – Complex glycan contactin-2 ectodomain, 1 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	9.3	nm
D_max	45.0	nm
Volume^Porod	780	nm³

SASDG49 – Apolipoprotein E3

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.6	nm

SASDN49 – Complex glycan contactin-2 ectodomain, 2 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	10.3	nm
D_max	60.0	nm
Volume^Porod	1290	nm³

SASDG59 – Apolipoprotein E4

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.8	nm

SASDN59 – Complex glycan contactin-2 ectodomain, 3 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	11.2	nm
D_max	60.0	nm
Volume^Porod	1540	nm³

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich experimental SAS data

Splicing factor, proline- and glutamine-rich Kratky plot

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20

Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C

R_g^Guinier	6.1	nm
D_max	22.0	nm
Volume^Porod	181	nm³

SASDG69 – Apolipoprotein E4 (K143A K146A) mutant

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data

Apolipoprotein E4 (K143A K146A) mutant Kratky plot

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.7	nm

SASDN69 – Complex glycan contactin-2 ectodomain, 6 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	11.0	nm
D_max	70.0	nm
Volume^Porod	1900	nm³

SASDG79 – Apolipoprotein E4 (methylated)

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.3	nm

SASDN79 – Complex glycan contactin-2 ectodomain, 12 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	11.3	nm
D_max	66.0	nm
Volume^Porod	1820	nm³

SASDG89 – Truncated Apolipoprotein E4 (1-191)

Apolipoprotein E4 (1-191) experimental SAS data

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.4	nm
D_max	7.7	nm

SASDN89 – Complex glycan contactin-2 ectodomain, 23 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	12.5	nm
D_max	65.0	nm
Volume^Porod	2044	nm³

SASDG99 – Apolipoprotein E2 (SEC-SAXS)

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.8	nm
D_max	20.0	nm

SASDN99 – Complex glycan contactin-2 ectodomain, 50 μM

Sample:	Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer:	25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	14.2	nm
D_max	65.0	nm
Volume^Porod	2190	nm³

SASDGA9 – Apolipoprotein E2 bound to 1 mg/mL heparin (SEC-SAXS)

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	21.0	nm

SASDGB9 – Apolipoprotein E2 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E2Suramin experimental SAS data

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.1	nm
D_max	18.3	nm

SASDGC9 – Apolipoprotein E3 (SEC-SAXS)

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.8	nm
D_max	20.0	nm

SASDGD9 – Apolipoprotein E3 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E3Heparin experimental SAS data

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	21.3	nm

SASDGE9 – Apolipoprotein E3 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E3Suramin experimental SAS data

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.1	nm
D_max	19.3	nm

SASDGF9 – Apolipoprotein E4 (SEC-SAXS)

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.9	nm
D_max	20.0	nm

SASDGG9 – Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	21.6	nm

SASDGH9 – Apolipoprotein E4 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.6	nm
D_max	18.7	nm

SASDGJ9 – Apolipoprotein E4 (methylated) - (SEC-SAXS)

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.9	nm
D_max	20.0	nm

SASDGK9 – Apolipoprotein E4 (methylated) bound to 1 mg/mL heparin (SEC-SAXS)

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.4	nm
D_max	21.7	nm

SASDGL9 – Apolipoprotein E4 (methylated) bound to 1 mM Suramin (SEC-SAXS)

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.3	nm
D_max	18.5	nm

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.8	nm
D_max	20.2	nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data

Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.7	nm
D_max	19.8	nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data

Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.4	nm
D_max	18.3	nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS)

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.3	nm
D_max	7.6	nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data

Heparin Apolipoprotein E4 (1-191) Kratky plot

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	4.2	nm
D_max	16.8	nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.4	nm
D_max	8.3	nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.7	nm
D_max	8.3	nm

SASDFY7 – Aquifex aeolicus McoA metaloxidase

Aquifex aeolicus McoA metaloxidase experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Apr 15

The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Lucas M, Monza E, Frazão C, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	7.5	nm
Volume^Porod	79	nm³

SASDFX7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆337-346 (MCoA∆337-346)

Aquifex aeolicus McoA metaloxidase ∆337-346 experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆337-346 monomer, 54 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Dec 4

The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Lucas M, Monza E, Frazão C, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	7.0	nm
Volume^Porod	78	nm³

SASDFW7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆328-352 (MCoA∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 13

The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Lucas M, Monza E, Frazão C, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	6.9	nm
Volume^Porod	77	nm³

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