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13 hits found for Gräwert

SASDJ93 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type apo-form

Ubiquitin carboxyl-terminal hydrolase MINDY-2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 45 nm3

SASDJA3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type bound to mono-ubiquitin

Ubiquitin carboxyl-terminal hydrolase MINDY-2Polyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.3 nm
Dmax 6.8 nm
VolumePorod 58 nm3

SASDJB3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to di-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.5 nm
Dmax 8.0 nm
VolumePorod 68 nm3

SASDJC3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C trimer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 8.8 nm
VolumePorod 97 nm3

SASDJD3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tetra-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C tetramer, 34 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.9 nm
Dmax 9.2 nm
VolumePorod 111 nm3

SASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C pentamer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 9.0 nm
VolumePorod 116 nm3

SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data
Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 8
Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...Gräwert TW, Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 50 nm3

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data
DAMMIN model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 37 nm3

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3