Small Angle Scattering Biological Data Bank SASBDB


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4 hits found for Parihar

SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H)Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform experimental SAS data

Sample:	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) monomer, 70 kDa Homo sapiens protein Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein
Buffer:	25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20

B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations. Proc Natl Acad Sci U S A 121(1):e2310727120 (2024)
...Parihar PS, Konovolov K, Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y

R_g^Guinier	4.0	nm
D_max	12.0	nm
Volume^Porod	266	nm³

SASDTX2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme E198K mutant

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) experimental SAS data

Sample:	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) monomer, 70 kDa Homo sapiens protein
Buffer:	25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20

R_g^Guinier	4.2	nm
D_max	14.0	nm
Volume^Porod	305	nm³

SASDK46 – Coronin from Trypanosoma brucei brucei (C-terminal coiled coil domain)

Sample:	Coronin tetramer, 21 kDa Trypanosoma brucei brucei … protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 17

Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction Current Research in Structural Biology (2021)
Parihar P, Singh A, Karade S, Sahasrabuddhe A, Pratap J

R_g^Guinier	3.3	nm
D_max	12.5	nm
Volume^Porod	22	nm³

SASDK56 – Cell cycle associated protein MOB1, putative, from Leishmania donovani

Cell cycle associated protein MOB1, putative experimental SAS data

Sample:	Cell cycle associated protein MOB1, putative monomer, 27 kDa Leishmania donovani (strain … protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2017 Jun 22

R_g^Guinier	2.1	nm
D_max	5.6	nm
Volume^Porod	59	nm³

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SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype

SASDTX2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme E198K mutant

SASDK46 – Coronin from Trypanosoma brucei brucei (C-terminal coiled coil domain)

SASDK56 – Cell cycle associated protein MOB1, putative, from Leishmania donovani