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7 hits found for AFFFF

SASDW94 – Horse apoferritin from AF4-SAXS measurements

Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 480 kDa Equus caballus protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19
AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline
Melissa Graewert
RgGuinier 5.4 nm
Dmax 13.3 nm
VolumePorod 632 nm3

SASDWA4 – Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements

Alcohol dehydrogenase 1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein
Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13
AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline
Melissa Graewert
RgGuinier 3.4 nm
Dmax 9.5 nm
VolumePorod 197 nm3

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement

Beta-amylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13
AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline
Melissa Graewert
RgGuinier 4.2 nm
Dmax 12.6 nm
VolumePorod 297 nm3

SASDWC4 – 100 nm polystyrene beads from AF4-SAXS measurements

Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm experimental SAS data
Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm Kratky plot
Sample: Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct
Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19
AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline
Melissa Graewert
RgGuinier 47.7 nm
Dmax 111.0 nm

SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm experimental SAS data
Thermo Fisher polystyrene nanospheres with a diameter of 20 nm Kratky plot
Sample: Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct
Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19
AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline
Melissa Graewert
RgGuinier 9.0 nm
Dmax 25.0 nm

SASDSH7 – Cationic liposomes containing DOTMA:DOPE (2:1) with negatively charged messenger RNA (mRNA) (raito 0.65:1)

mRNA -- proprietary sequence(R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride1,2-dioleoyl-sn-glycero-3-phosphoethanolamine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: mRNA -- proprietary sequence , 400 kDa RNA
(R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride , 1 kDa lipid
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine , 1 kDa lipid
Buffer: 10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jun 9
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

SASDRQ8 – ...AFFFF)

Albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: 10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 104 nm3