SASBDB entries for UniProt ID:

SASDHR8 – Mosquito-larvicidal binary toxin receptor (Cqm1) protein

UniProt ID: B0X223 (23-560) Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT)
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) dimer, 129 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 4.0 nm
Dmax 9.2 nm
VolumePorod 97 nm3

SASDHS8 – Mosquito-larvicidal BinB protein

UniProt ID: P10565 (1-448) Binary larvicide subunit BinB
Binary larvicide subunit BinB experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 48 nm3

SASDHT8 – Complex of mosquito-larvicidal BinB protein and the Mosquito-larvicidal binary toxin receptor (Cqm1)

UniProt ID: P10565 (1-448) Binary larvicide subunit BinB

UniProt ID: B0X223 (23-560) Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT)
Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment: SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 4.6 nm
Dmax 11.7 nm
VolumePorod 290 nm3

SASDHU8 – SANS study of the complex between mosquito-larvicidal BinB protein and the mosquito-larvicidal binary toxin receptor (Cqm1)

UniProt ID: P10565 (1-448) Binary larvicide subunit BinB

UniProt ID: B0X223 (23-560) Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT)
Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data
OTHER model
Sample: Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 26
Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
Sharma M, Kumar A, Kumar V
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 174 nm3

SASDHV8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 267 nm3

SASDHW8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 274 nm3

SASDHX8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 258 nm3

SASDHY8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 5.0 nm
Dmax 17.4 nm
VolumePorod 270 nm3

SASDHZ8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM TRIS, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 239 nm3

SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM phosphate, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 240 nm3