SASBDB entries for UniProt ID:

SASDHN7 – Emfourin (M4in) from Serratia proteamaculans, M4 family peptidase inhibitor

UniProt ID: A0A1X9WII3 (1-113) Emfourin (Protealysin-associated protein)
Emfourin (Protealysin-associated protein) experimental SAS data
DAMFILT model
Sample: Emfourin (Protealysin-associated protein) monomer, 13 kDa Serratia proteamaculans protein
Buffer: 50 mM Tris-HCL, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Apr 22
NMR structure of emfourin, a novel protein metalloprotease inhibitor: insights into the mechanism of action. J Biol Chem :104585 (2023)
Bozin T, Berdyshev I, Chukhontseva K, Karaseva M, Konarev P, Varizhuk A, Lesovoy D, Arseniev A, Kostrov S, Bocharov E, Demidyuk I
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 16 nm3

SASDH28 – Mitochondrial import inner membrane translocase TIM 9·10·12

UniProt ID: P32830 (2-109) Mitochondrial import inner membrane translocase subunit TIM12

UniProt ID: O74700 (1-87) Mitochondrial import inner membrane translocase subunit TIM9

UniProt ID: P87108 (2-93) Mitochondrial import inner membrane translocase subunit TIM10
Mitochondrial import inner membrane translocase subunit TIM12Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM12 monomer, 12 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM9 dimer, 20 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM MES buffer (2-(N-morpholino)ethanesulfonic acid), pH 6.5, 50 mM NaCl Cloning,, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 8
Architecture and subunit dynamics of the mitochondrial TIM9·10·12 chaperone (2020)
Weinhäupl K, Wang Y, Hessel A, Brennich M, Lindorff-Larsen K, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDHF8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

UniProt ID: P15516 (20-43) Histatin-3, His3-(20-43)-peptide
Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 4.89 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K Rg histogram
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.4 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDHG8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 2.51 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

UniProt ID: P15516 (20-43) Histatin-3, His3-(20-43)-peptide
Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.4 nm
VolumePorod 3 nm3

SASDHH8 – Histatin 5 (Histatin 3; His3-(20-43)-peptide) at 1.26 mg/ml in 20 mM Tris 150 mM NaCl pH 7.0, 298 K

UniProt ID: P15516 (20-43) Histatin-3, His3-(20-43)-peptide
Histatin-3, His3-(20-43)-peptide experimental SAS data
Histatin-3, His3-(20-43)-peptide Kratky plot
Sample: Histatin-3, His3-(20-43)-peptide monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 13
Comment on the Optimal Parameters to Derive Intrinsically Disordered Protein Conformational Ensembles from Small-Angle X-ray Scattering Data Using the Ensemble Optimization Method Journal of Chemical Theory and Computation (2021)
Sagar A, Jeffries C, Petoukhov M, Svergun D, Bernadó P
RgGuinier 1.5 nm
Dmax 6.0 nm
VolumePorod 3 nm3

SASDHJ8 – ACT domain of the Mycobacterium tuberculosis Rel protein

UniProt ID: P9WHG9 (660-738) ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA)
ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) experimental SAS data
ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) Kratky plot
Sample: ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) dimer, 20 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 350 mM NaCl, 5% glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at Bruker Nanostar, Nanyang Technological University on 2018 Jun 7
Atomic structure of, and valine binding to the regulatory ACT domain of the Mycobacterium tuberculosis Rel protein. FEBS J (2020)
Shin J, Singal B, Manimekalai MSS, Chen MW, Ragunathan P, Grüber G
RgGuinier 1.9 nm
Dmax 6.1 nm
VolumePorod 29 nm3

SASDHL8 – Aquifex aeolicus McoA metaloxidase evolved variant (2F4)

UniProt ID: O67206 (44-527) McoA evolved variant 2F4 (Periplasmic cell division protein (SufI))
McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) experimental SAS data
OTHER model
Sample: McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) monomer, 55 kDa Aquifex aeolicus VF5 protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 25
Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase ACS Catalysis :5022-5035 (2022)
Brissos V, Borges P, Núñez-Franco R, Lucas M, Frazão C, Monza E, Masgrau L, Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 6.8 nm
VolumePorod 78 nm3

SASDHN8 – Human tumor necrosis factor receptor superfamily member 5 (CD40) extracellular domain in complex with human immunoglobulin gamma 1 (IgG1) 341G2 F(ab)

UniProt ID: P25942 (21-193) Tumor necrosis factor receptor superfamily member 5

UniProt ID: None (None-None) Human 341G2 F(ab)
Tumor necrosis factor receptor superfamily member 5Human 341G2 F(ab) experimental SAS data
HADDOCK model
Sample: Tumor necrosis factor receptor superfamily member 5 monomer, 19 kDa Homo sapiens protein
Human 341G2 F(ab) monomer, 73 kDa Homo sapiens protein
Buffer: Phosphate-buffered saline, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18
Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity Cancer Cell (2020)
Yu X, Chan H, Fisher H, Penfold C, Kim J, Inzhelevskaya T, Mockridge C, French R, Duriez P, Douglas L, English V, Verbeek J, White A, Tews I, Glennie M, Cragg M
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 97 nm3

SASDHP8 – Pentafunctional AROM polypeptide from Chaetomium thermophilum

UniProt ID: G0S061 (1-1581) Pentafunctional AROM polypeptide
Pentafunctional AROM polypeptide experimental SAS data
Pentafunctional AROM polypeptide Kratky plot
Sample: Pentafunctional AROM polypeptide dimer, 345 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 150 mM NaCl. 2 mM TCEP, 1% sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Nov 22
Architecture and functional dynamics of the pentafunctional AROM complex Nature Chemical Biology (2020)
Arora Verasztó H, Logotheti M, Albrecht R, Leitner A, Zhu H, Hartmann M
RgGuinier 5.4 nm
Dmax 17.5 nm
VolumePorod 623 nm3

SASDHQ8 – Severe fever with thrombocytopenia syndrome virus (SFTSV) full-length L protein

UniProt ID: F1BV96 (1-2084) Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase experimental SAS data
Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase Kratky plot
Sample: Severe fever with thrombocytopenia syndrome virus L protein - RNA-dependent RNA polymerase monomer, 238 kDa SFTS virus AH12 protein
Buffer: 50 mM HEPES(NaOH) pH 7.0, 500 mM NaCl, 5% (w/v) glycerol, and 2 mM dithiothreitol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 13
Structural and functional characterization of the Severe fever with thrombocytopenia syndrome virus L protein (2020)
Vogel D, Thorkelsson S, Quemin E, Meier K, Kouba T, Gogrefe N, Busch C, Reindl S, Günther S, Cusack S, Grünewald K, Rosenthal M
RgGuinier 4.5 nm
Dmax 14.3 nm
VolumePorod 483 nm3