SASBDB entries for UniProt ID:

SASDJE2 – Beta-ketoacyl synthase (Bamb_5925) C-terminal docking domain

UniProt ID: Q0B303 (2616-2644) Beta-ketoacyl synthase Bamb_5925
Beta-ketoacyl synthase Bamb_5925 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5925 monomer, 4 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 3 nm3

SASDJF2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain

UniProt ID: Q0B304 (1-41) Beta-ketoacyl synthase Bamb_5924
Beta-ketoacyl synthase Bamb_5924 experimental SAS data
DAMFILT model
Sample: Beta-ketoacyl synthase Bamb_5924 dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 6
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.9 nm
Dmax 7.5 nm
VolumePorod 22 nm3

SASDJG2 – Beta-ketoacyl synthase (Bamb_5920) C-terminal docking domain

UniProt ID: Q0B308 (2956-2975) Beta-ketoacyl synthase Bamb_5920
Beta-ketoacyl synthase Bamb_5920 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5920 monomer, 2 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.2 nm
Dmax 4.9 nm
VolumePorod 3 nm3

SASDJH2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain mutant K32A

UniProt ID: Q0B304 (1-41) Beta-ketoacyl synthase Bamb_5924, K32A mutant
Beta-ketoacyl synthase Bamb_5924, K32A mutant experimental SAS data
Beta-ketoacyl synthase Bamb_5924, K32A mutant Kratky plot
Sample: Beta-ketoacyl synthase Bamb_5924, K32A mutant dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.8 nm
Dmax 8.0 nm
VolumePorod 22 nm3

SASDJJ2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain mutant R17A

UniProt ID: Q0B304 (1-41) Beta-ketoacyl synthase Bamb_5924, R17A mutant
Beta-ketoacyl synthase Bamb_5924, R17A mutant experimental SAS data
Beta-ketoacyl synthase Bamb_5924, R17A mutant Kratky plot
Sample: Beta-ketoacyl synthase Bamb_5924, R17A mutant dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol :107581 (2020)
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
RgGuinier 1.8 nm
Dmax 8.2 nm
VolumePorod 20 nm3

SASDJM2 – Flagellotropic bacteriophage YSD1 protein, YSD1_22 - Major Tail Protein

UniProt ID: M9NUS9 (1-381) Major tail protein
Major tail protein experimental SAS data
DAMFILT model
Sample: Major tail protein monomer, 40 kDa Salmonella virus Chi protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 8
The architecture and stabilisation of flagellotropic tailed bacteriophages. Nat Commun 11(1):3748 (2020)
Hardy JM, Dunstan RA, Grinter R, Belousoff MJ, Wang J, Pickard D, Venugopal H, Dougan G, Lithgow T, Coulibaly F
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 54 nm3

SASDJT2 – DNA-directed RNA polymerase (RPO41)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase
DNA-directed RNA polymerase experimental SAS data
DNA-directed RNA polymerase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.4 nm
Dmax 12.5 nm
VolumePorod 295 nm3

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase

UniProt ID: Q8ILT8 (43-381) rRNA adenine N (6) methyl transferase
DNA-directed RNA polymeraserRNA adenine N (6) methyl transferase experimental SAS data
DNA-directed RNA polymerase rRNA adenine N (6) methyl transferase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
RRNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 5.5 nm
Dmax 15.0 nm
VolumePorod 393 nm3

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1)

UniProt ID: P13433 (30-1351) DNA-directed RNA polymerase

UniProt ID: P14908 (1-341) Mitochondrial transcription factor 1
DNA-directed RNA polymeraseMitochondrial transcription factor 1 experimental SAS data
DNA-directed RNA polymerase Mitochondrial transcription factor 1 Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.5 nm
Dmax 14.4 nm
VolumePorod 292 nm3

SASDJW2 – Modification dependent EcoKMcrA restriction endonuclease

UniProt ID: P24200 (1-277) 5-methylcytosine-specific restriction enzyme A
5-methylcytosine-specific restriction enzyme A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 5-methylcytosine-specific restriction enzyme A dimer, 65 kDa Escherichia coli protein
Buffer: 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 May 24
Activity and structure of EcoKMcrA. Nucleic Acids Res 46(18):9829-9841 (2018)
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 95 nm3