SASBDB entries for UniProt ID:

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin

UniProt ID: P01308 (25-110) Insulin (Insulin B chain and Insulin A chain)

UniProt ID: None (None-None) HUI-018 Fab
Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration

UniProt ID: P01308 (25-110) Insulin (Insulin B chain and Insulin A chain)

UniProt ID: None (None-None) HUI-018 Fab
Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 105 nm3

SASDJ24 – Immunoglobulin HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin

UniProt ID: None (None-None) HUI-018 Fab

UniProt ID: P01315 (25-108) Insulin (Insulin B chain and Insulin A chain)

UniProt ID: None (None-None) OXI-005 Fab
HUI-018 FabInsulin (Insulin B chain and Insulin A chain)OXI-005 Fab experimental SAS data
OTHER model
Sample: HUI-018 Fab monomer, 47 kDa Mus musculus protein
Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Sus scrofa protein
OXI-005 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2019 Mar 28
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 4.5 nm
Dmax 14.2 nm
VolumePorod 138 nm3

SASDJ34 – Human Complex I assembly factor ACAD9 dimer

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial
Complex I assembly factor ACAD9, mitochondrial experimental SAS data
CUSTOM IN-HOUSE model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 213 nm3

SASDJ44 – Human Complex I assembly factor ACAD9 complexed with ECSIT

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial

UniProt ID: Q9BQ95 (49-431) Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrial experimental SAS data
DAMFILT model
Sample: Complex I assembly factor ACAD9, mitochondrial dimer, 130 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial dimer, 93 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 5.1 nm
Dmax 16.8 nm
VolumePorod 514 nm3

SASDJ54 – Human Complex I assembly factor ACAD9 complexed with ECSIT and SMT3-NDUFAF1

UniProt ID: Q9H845 (38-621) Complex I assembly factor ACAD9, mitochondrial

UniProt ID: Q9BQ95 (49-431) Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial

UniProt ID: Q9Y375 (25-327) Complex I intermediate-associated protein 30, mitochondrial
Complex I assembly factor ACAD9, mitochondrialEvolutionarily conserved signaling intermediate in Toll pathway, mitochondrialComplex I intermediate-associated protein 30, mitochondrial experimental SAS data
DAMMIF model
Sample: Complex I assembly factor ACAD9, mitochondrial tetramer, 259 kDa Homo sapiens protein
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial tetramer, 184 kDa Homo sapiens protein
Complex I intermediate-associated protein 30, mitochondrial tetramer, 190 kDa Homo sapiens protein
Buffer: 25mM Tris-HCl, 150mM NaCl, 0.1mM DTT, and 5% glycerol, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 2
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 24(10):103153 (2021)
Xia C, Lou B, Fu Z, Mohsen A, Shen A, Vockley J, Kim J
RgGuinier 6.6 nm
Dmax 21.6 nm
VolumePorod 1340 nm3

SASDJ64 – Calcium bound Calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1
Calmodulin-1 experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 27 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1

UniProt ID: P0DKX7 (454-484) Bifunctional hemolysin/adenylate cyclase
Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDJ84 – Intimin D00-D0 domain

UniProt ID: P19809 (450-650) Intimin, D00-D0 domain (6xHis tagged)
Intimin, D00-D0 domain (6xHis tagged) experimental SAS data
EOM/RANCH model
Sample: Intimin, D00-D0 domain (6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer: 10 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12
The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action. Sci Rep 10(1):21249 (2020)
Weikum J, Kulakova A, Tesei G, Yoshimoto S, Jægerum LV, Schütz M, Hori K, Skepö M, Harris P, Leo JC, Morth JP
RgGuinier 3.1 nm
Dmax 8.3 nm
VolumePorod 32 nm3

SASDJ94 – Intimin D0-D1 domain

UniProt ID: P19809 (553-752) Intimin (D0-D1 domain, 6xHis tagged)
Intimin (D0-D1 domain, 6xHis tagged) experimental SAS data
Intimin D0-D1 domain Rg histogram
Sample: Intimin (D0-D1 domain, 6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer: 10 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12
The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action. Sci Rep 10(1):21249 (2020)
Weikum J, Kulakova A, Tesei G, Yoshimoto S, Jægerum LV, Schütz M, Hori K, Skepö M, Harris P, Leo JC, Morth JP
RgGuinier 2.2 nm
Dmax 5.8 nm
VolumePorod 34 nm3