SASBDB entries for UniProt ID:

SASDJY8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

UniProt ID: Q8TB36 (1-318) Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
Nguyen G, Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P
RgGuinier 3.1 nm
Dmax 9.6 nm
VolumePorod 121 nm3

SASDJZ8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

UniProt ID: Q8TB36 (1-318) Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
Nguyen G, Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P
RgGuinier 2.7 nm
Dmax 9.0 nm
VolumePorod 69 nm3

SASDJ29 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

UniProt ID: Q8TB36 (1-318) Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358
Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data
GASBOR model
Sample: Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 11
Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
Nguyen G, Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P
RgGuinier 2.5 nm
Dmax 8.6 nm
VolumePorod 65 nm3

SASDJ39 – Monomeric human ganglioside-induced differentiation-associated protein 1-like 1, GDAP1L1

UniProt ID: Q96MZ0 (None-None) Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform)
Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) experimental SAS data
CORAL model
Sample: Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM TRIS pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 10
Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
Nguyen G, Sutinen A, Raasakka A, Muruganandam G, Loris R, Kursula P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 73 nm3

SASDJ49 – Apo PigH ACP1-ACP2

UniProt ID: Q5W264 (1-189) 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH
4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Apo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 83 nm3

SASDJ59 – Holo PigH ACP1-ACP2

UniProt ID: Q5W264 (1-189) 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH
4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Holo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 72 nm3

SASDJ69 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 150 mM NaCl studied with SAXS

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 8
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 7.6 nm
Dmax 41.0 nm

SASDJ79 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 150 mM NaCl studied with SANS

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 8.9 nm
Dmax 39.0 nm

SASDJ89 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 1400 mM NaCl studied with SANS

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
GASBOR model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 8.6 nm
Dmax 39.0 nm

SASDJ99 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 2800 mM NaCl studied with SANS

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
GASBOR model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 2800 mM NaCl, 76.6 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 9.0 nm
Dmax 39.0 nm