SASBDB entries for UniProt ID:

SASDL33 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with paused-flow SEC-SANS

UniProt ID: Q7NDN8 (44-359) Proton-gated ion channel
Proton-gated ion channel experimental SAS data
GROMACS model
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 274 nm3

SASDL43 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 3.0 measured with paused-flow SEC-SANS

UniProt ID: Q7NDN8 (44-359) Proton-gated ion channel
Proton-gated ion channel experimental SAS data
GROMACS model
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM citrate, 150 mM NaCl, 0.5 mM match-out deuterated DDM, pH: 3
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.7 nm
VolumePorod 279 nm3

SASDL53 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with continuous-flow SEC-SANS

UniProt ID: Q7NDN8 (44-359) Proton-gated ion channel
Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 20
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.0 nm
VolumePorod 235 nm3

SASDL63 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with cuvette-mode SANS

UniProt ID: Q7NDN8 (44-359) Proton-gated ion channel
Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 21
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
Lycksell M, Rovšnik U, Bergh C, Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 225 nm3

SASDLF3 – Staphylococcal immunoglobulin-binding protein bound to complement component C3

UniProt ID: Q70AB8 (28-266) Protein A
Protein A experimental SAS data
DAMMIN model
Sample: Protein A monomer, 28 kDa Staphylococcus aureus protein
Buffer: 10 mM HEPES, pH 7.2, 3 mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 12
A structural basis for Staphylococcal complement subversion: X-ray structure of the complement-binding domain of Staphylococcus aureus protein Sbi in complex with ligand C3d Molecular Immunology 48(4):452-462 (2011)
Clark E, Crennell S, Upadhyay A, Zozulya A, Mackay J, Svergun D, Bagby S, van den Elsen J
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 81 nm3

SASDLG3 – Thermoplasma E2 catalytic core

UniProt ID: P03120 (177-400) Regulatory protein E2
Regulatory protein E2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Regulatory protein E2, 1037 kDa Human papillomavirus type … protein
Buffer: 50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 13
The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly FEBS Journal 279(5):713-723 (2012)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, Danson M, van den Elsen J
RgGuinier 8.8 nm
Dmax 22.0 nm
VolumePorod 2473 nm3

SASDLH3 – Truncated Thermoplasma E2 catalytic core

UniProt ID: P03120 (182-400) Regulatory protein E2
Regulatory protein E2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Regulatory protein E2 trimer, 73 kDa Human papillomavirus type … protein
Buffer: 50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 27
Why are the 2-oxoacid dehydrogenase complexes so large? Generation of an active trimeric complex Biochemical Journal 463(3):405-412 (2014)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, van den Elsen J, Danson M
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 149 nm3

SASDLJ3 – SH3 and multiple ankyrin repeat domains protein 3 (wild type)

UniProt ID: Q9JLU4 (1-676) SH3 and multiple ankyrin repeat domains protein 3
SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses. Elife 10 (2021)
Bucher M, Niebling S, Han Y, Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 170 nm3

SASDLK3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (L68P)

UniProt ID: Q9JLU4 (1-676) SH3 and multiple ankyrin repeat domains protein 3
SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses. Elife 10 (2021)
Bucher M, Niebling S, Han Y, Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M
RgGuinier 4.1 nm
Dmax 14.8 nm
VolumePorod 224 nm3

SASDLL3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (R12C)

UniProt ID: Q9JLU4 (1-676) SH3 and multiple ankyrin repeat domains protein 3
SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data
CORAL model
Sample: SH3 and multiple ankyrin repeat domains protein 3 monomer, 87 kDa Rattus norvegicus protein
Buffer: 100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3
Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses. Elife 10 (2021)
Bucher M, Niebling S, Han Y, Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 175 nm3