SASBDB entries for UniProt ID:

SASDN29 – Complex glycan contactin-2 immunoglobulin domains1-6, 51μM

UniProt ID: Q61330 (29-608) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 7
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.8 nm
Dmax 32.0 nm
VolumePorod 412 nm3

SASDN39 – Complex glycan contactin-2 ectodomain, 1 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 9.3 nm
Dmax 45.0 nm
VolumePorod 780 nm3

SASDN49 – Complex glycan contactin-2 ectodomain, 2 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 10.3 nm
Dmax 60.0 nm
VolumePorod 1290 nm3

SASDN59 – Complex glycan contactin-2 ectodomain, 3 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.2 nm
Dmax 60.0 nm
VolumePorod 1540 nm3

SASDN69 – Complex glycan contactin-2 ectodomain, 6 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.0 nm
Dmax 70.0 nm
VolumePorod 1900 nm3

SASDN79 – Complex glycan contactin-2 ectodomain, 12 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.3 nm
Dmax 66.0 nm
VolumePorod 1820 nm3

SASDN89 – Complex glycan contactin-2 ectodomain, 23 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 12.5 nm
Dmax 65.0 nm
VolumePorod 2044 nm3

SASDN99 – Complex glycan contactin-2 ectodomain, 50 μM

UniProt ID: Q61330 (29-1004) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 14.2 nm
Dmax 65.0 nm
VolumePorod 2190 nm3

SASDNA9 – Acinetobacter baumannii putrescine N-hydroxylase, 1 mg/mL

UniProt ID: A0A0J0ZLX8 (1-446) L-lysine 6-monooxygenase (NADPH-requiring)
L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 394 nm3

SASDNB9 – Acinetobacter baumannii putrescine N-hydroxylase, 3 mg/mL

UniProt ID: A0A0J0ZLX8 (1-446) L-lysine 6-monooxygenase (NADPH-requiring)
L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 390 nm3