SASBDB entries for UniProt ID:

SASDPY2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein monomer

UniProt ID: P0DTD1 (3860-4140) Replicase polyprotein 1ab
Replicase polyprotein 1ab experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 50 nm3

SASDPZ2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein dimer

UniProt ID: P0DTD1 (3860-4140) Replicase polyprotein 1ab
Replicase polyprotein 1ab experimental SAS data
Replicase polyprotein 1ab Kratky plot
Sample: Replicase polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 110 nm3

SASDP23 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein monomer

UniProt ID: P0DTC1 (3860-4405) Replicase polyprotein 1a
Replicase polyprotein 1a experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.5 nm
Dmax 15.6 nm
VolumePorod 102 nm3

SASDP33 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein dimer

UniProt ID: P0DTC1 (3860-4405) Replicase polyprotein 1a
Replicase polyprotein 1a experimental SAS data
Replicase polyprotein 1a Kratky plot
Sample: Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 4.6 nm
Dmax 19.1 nm
VolumePorod 203 nm3

SASDP43 – Accumulation-association protein (Aap) Brpt5.5 monomer

UniProt ID: Q5HKE8 (1505-2223) Accumulation associated protein
Accumulation associated protein experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 monomer Rg histogram
Sample: Accumulation associated protein monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 16
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.7 nm
Dmax 55.8 nm

SASDP53 – Accumulation-association protein (Aap) Brpt5.5 5xH85A monomer

UniProt ID: Q5HKE8 (1505-2223) Accumulation associated protein (mutant)
Accumulation associated protein (mutant) experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 5xH85A monomer Rg histogram
Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.1 nm
Dmax 57.2 nm

SASDP63 – Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer

UniProt ID: Q5HKE8 (1505-2223) Accumulation associated protein (mutant)
Accumulation associated protein (mutant) experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer Rg histogram
Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.4 nm
Dmax 56.6 nm

SASDP73 – Accumulation-association protein (Aap) Brpt5.5 5xH85A dimer in the presence of ZnCl2

UniProt ID: Q5HKE8 (1505-2223) Accumulation associated protein (mutant)
Accumulation associated protein (mutant) experimental SAS data
PYMOL model
Sample: Accumulation associated protein (mutant) dimer, 155 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 15.0 nm
Dmax 63.3 nm

SASDP83 – Accumulation-association protein (Aap) Brpt5.5 2xH85A tetramer in the presence of ZnCl2

UniProt ID: Q5HKE8 (1505-2223) Accumulation associated protein (mutant)
Accumulation associated protein (mutant) experimental SAS data
PYMOL model
Sample: Accumulation associated protein (mutant) tetramer, 312 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 15.7 nm
Dmax 70.0 nm

SASDP93 – K48-linked tetraubiquitin (Ub4)

UniProt ID: P0CG47 (1-76) Polyubiquitin-B
Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 2.6 nm
Dmax 95.0 nm
VolumePorod 44 nm3