Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (1154-1720) Polyketide synthase Pks13

Polyketide synthase Pks13 experimental SAS data

Sample:	Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	80	nm³

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (576-1063) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDNQ9 – Acyltransferase domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (591-1046) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Jun 3

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	85	nm³

SASDNR9 – Subfragment ACP1KSAT tridomain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (1-1063) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 116 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 8.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.8	nm
D_max	12.9	nm
Volume^Porod	186	nm³

SASDNS9 – KS domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (119-575) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Dec 7

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

SASDNT9 – Subfragment KSAT of Polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (119-1070) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 104 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2008 Sep 20

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	155	nm³

SASDNU9 – Mycocerosic acid synthase in its apo form and at neutral pH

UniProt ID: Q02251 (1-2111) Mycocerosic acid synthase

Mycocerosic acid synthase experimental SAS data

Sample:	Mycocerosic acid synthase monomer, 224 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Oct 1

R_g^Guinier	5.7	nm
D_max	21.0	nm
Volume^Porod	420	nm³

SASDNV9 – Phenolpthiocerol synthase A of BCG at neutral pH

UniProt ID: A0A0H3M7U0 (1-1876) Phenolpthiocerol synthesis type-I polyketide synthase ppsA

Phenolpthiocerol synthesis type-I polyketide synthase ppsA experimental SAS data

Sample:	Phenolpthiocerol synthesis type-I polyketide synthase ppsA monomer, 199 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Jun 14

R_g^Guinier	6.7	nm
D_max	20.2	nm
Volume^Porod	680	nm³

SASDPE2 – Starch adherence system protein 6 (Sas6)

UniProt ID: A0A2N0UYM2 (31-665) Dockerin type I repeat

Dockerin type I repeat experimental SAS data

Sample:	Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer:	phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
Photenhauer AL, Villafuerte-Vega RC, Cerqueira FM, Armbruster KM, Mareček F, Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM

R_g^Guinier	3.0	nm
D_max	9.0	nm
Volume^Porod	97	nm³

SASDPF2 – Structure of an extracellular superoxide dismutase from Onchocerca volvulus

UniProt ID: Q07449 (44-201) Extracellular superoxide dismutase [Cu-Zn]

Extracellular superoxide dismutase [Cu-Zn] experimental SAS data

Sample:	Extracellular superoxide dismutase [Cu-Zn] dimer, 34 kDa Onchocerca volvulus protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13

Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development Acta Crystallographica Section F Structural Biology Communications 78(6) (2022)
Moustafa A, Perbandt M, Liebau E, Betzel C, Falke S

R_g^Guinier	2.6	nm
D_max	9.0	nm
Volume^Porod	55	nm³

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