SASBDB entries for UniProt ID:

SASDP87 – Bovine serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

UniProt ID: P02769 (1-607) Albumin
Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 108 nm3

SASDP97 – Bovine serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

UniProt ID: P02769 (1-607) Albumin
Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 104 nm3

SASDPA7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 5 MPa (1 atm)

UniProt ID: P39687 (1-249) Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant)
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 31 nm3

SASDPB7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 100 MPa (1000 atm)

UniProt ID: P39687 (1-249) Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant)
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.0 nm
Dmax 7.5 nm
VolumePorod 37 nm3

SASDPC7 – Ordered core SD1 domain of KNR4

UniProt ID: P32566 (80-340) Cell wall assembly regulator SMI1
Cell wall assembly regulator SMI1 experimental SAS data
DAMMIN model
Sample: Cell wall assembly regulator SMI1 monomer, 31 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 100 mM MES, 300 mM NaCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2016 Mar 18
The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein. J Mol Biol :168048 (2023)
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 62 nm3

SASDPD7 – C-terminal truncated KNR4

UniProt ID: P32566 (1-345) Cell wall assembly regulator SMI1
Cell wall assembly regulator SMI1 experimental SAS data
DAMMIN model
Sample: Cell wall assembly regulator SMI1 monomer, 40 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 100 mM MES, 300 mM NaCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 4
The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein. J Mol Biol :168048 (2023)
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D
RgGuinier 2.8 nm
Dmax 11.0 nm
VolumePorod 79 nm3

SASDPE7 – Full length KNR4

UniProt ID: P32566 (1-505) Cell wall assembly regulator SMI1
Cell wall assembly regulator SMI1 experimental SAS data
DAMMIN model
Sample: Cell wall assembly regulator SMI1 monomer, 57 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 100 mM MES, 300 mM NaCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2016 Mar 7
The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein. J Mol Biol :168048 (2023)
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 117 nm3

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: Q00511 (1-302) Urate Oxidase (Uricase) from Aspergillus flavus
Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 8.8 nm
VolumePorod 221 nm3