SASBDB entries for UniProt ID:

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: Q00511 (1-302) Urate Oxidase (Uricase) from Aspergillus flavus
Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 214 nm3

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: P24300 (1-388) Xylose isomerase
Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 14.1 nm
VolumePorod 228 nm3

SASDPL7 – Xylose isomerase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: P24300 (1-388) Xylose isomerase
Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.3 nm
Dmax 14.1 nm
VolumePorod 233 nm3

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: F8W669 (1-190) Endo-1,4-beta-xylanase
Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.8 nm
VolumePorod 25 nm3

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: F8W669 (1-190) Endo-1,4-beta-xylanase
Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDPP7 – Amyloid Beta 1-42 (cluster 1, Set 2, final state)

UniProt ID: P05067 (672-713) Amyloid-beta precursor protein
Amyloid-beta precursor protein experimental SAS data
DAMMIN model
Sample: Amyloid-beta precursor protein, 5 kDa Homo sapiens protein
Buffer: 1 mM Hepes, 0.12 % NH4OH, pH: 10.7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 11
Time-resolved small-angle X-ray scattering study of polymorphic nature of Aß42 oligomerisation
Tia Cheremnykh
RgGuinier 5.8 nm
Dmax 42.0 nm

SASDPQ7 – Amyloid Beta 1-42 (cluster 1, Set 3, final state)

UniProt ID: P05067 (672-713) Amyloid-beta precursor protein
Amyloid-beta precursor protein experimental SAS data
DAMMIN model
Sample: Amyloid-beta precursor protein, 5 kDa Homo sapiens protein
Buffer: 1 mM Hepes, 0.12 % NH4OH, pH: 10.7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 13
Time-resolved small-angle X-ray scattering study of polymorphic nature of Aß42 oligomerisation
Tia Cheremnykh
RgGuinier 6.9 nm
Dmax 60.0 nm

SASDPR7 – Amyloid Beta 1-42 (cluster 2, Set 4, initial state)

UniProt ID: P05067 (672-713) Amyloid-beta precursor protein
Amyloid-beta precursor protein experimental SAS data
DAMMIN model
Sample: Amyloid-beta precursor protein, 5 kDa Homo sapiens protein
Buffer: 1 mM Hepes, 0.1 % NH4OH (pH∼10.7), pH: 10.7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 31
Time-resolved small-angle X-ray scattering study of polymorphic nature of Aß42 oligomerisation
Tia Cheremnykh
RgGuinier 2.5 nm
Dmax 6.5 nm

SASDPS7 – Amyloid Beta 1-42 (cluster 2, Set 4, final state)

UniProt ID: P05067 (672-713) Amyloid-beta precursor protein
Amyloid-beta precursor protein experimental SAS data
DAMMIN model
Sample: Amyloid-beta precursor protein, 5 kDa Homo sapiens protein
Buffer: 1 mM Hepes, 0.1 % NH4OH (pH∼10.7), pH: 10.7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 1
Time-resolved small-angle X-ray scattering study of polymorphic nature of Aß42 oligomerisation
Tia Cheremnykh
RgGuinier 3.7 nm
Dmax 9.1 nm

SASDPT7 – Amyloid Beta 1-42 (cluster 2, Set 5, final state)

UniProt ID: P05067 (672-713) Amyloid-beta precursor protein
Amyloid-beta precursor protein experimental SAS data
DAMMIN model
Sample: Amyloid-beta precursor protein, 5 kDa Homo sapiens protein
Buffer: 1 mM Hepes, 0.1 % NH4OH (pH∼10.7), pH: 10.7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 1
Time-resolved small-angle X-ray scattering study of polymorphic nature of Aß42 oligomerisation
Tia Cheremnykh
RgGuinier 3.7 nm
Dmax 11.0 nm