SASBDB entries for UniProt ID:

SASDQ46 – Retinoic acid-induced protein 2 (RAI2: 303-465)

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465)
Retinoic acid-induced protein 2 (303-465) experimental SAS data
Retinoic acid-induced protein 2 (303-465) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 55 nm3

SASDQ56 – Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465: L319A, S320A)
Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data
Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 54 nm3

SASDQ66 – Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465: L345A, S346A)
Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data
Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 55 nm3

SASDQ76 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362)

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.4 nm
Dmax 25.0 nm
VolumePorod 889 nm3

SASDQ86 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: L319A, S320A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 368 nm3

SASDQ96 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: L345A, S346A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 5.5 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDQA6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1+M2 mutant L319A, S320A, L345A, S346A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 15.5 nm
VolumePorod 352 nm3

SASDQB6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) deletion mutant ΔE331-A341

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: ΔE331-A341)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: ΔE331-A341) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: ΔE331-A341) monomer, 6 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.2 nm
Dmax 28.0 nm
VolumePorod 912 nm3

SASDQC6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465)

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.4 nm
Dmax 28.0 nm
VolumePorod 978 nm3

SASDQD6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465: L319A, S320A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 18.5 nm
VolumePorod 316 nm3