SASBDB entries for UniProt ID:

SASDQS5 – Oligomerisation domain of phosphoprotein from Gaboon viper virus

UniProt ID: L0N429 (67-178) Phosphoprotein
Phosphoprotein experimental SAS data
DAMMIF model
Sample: Phosphoprotein tetramer, 52 kDa Gaboon viper virus … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM β- mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Sep 1
Borna Disease Virus 1 Phosphoprotein Forms a Tetramer and Interacts with Host Factors Involved in DNA Double-Strand Break Repair and mRNA Processing. Viruses 14(11) (2022)
Tarbouriech N, Chenavier F, Kawasaki J, Bachiri K, Bourhis JM, Legrand P, Freslon LL, Laurent EMN, Suberbielle E, Ruigrok RWH, Tomonaga K, Gonzalez-Dunia D, Horie M, Coyaud E, Crépin T
RgGuinier 4.6 nm
Dmax 17.5 nm
VolumePorod 75 nm3

SASDQT5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type

UniProt ID: Q01560 (120-280) Serine/arginine (SR)-type shuttling mRNA binding protein NPL3
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 2.0 nm
Dmax 6.8 nm
VolumePorod 24 nm3

SASDQU5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) P196D/A197D linker mutant

UniProt ID: Q01560 (120-280) Serine/arginine (SR)-type shuttling mRNA binding protein NPL3
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 1.9 nm
Dmax 6.7 nm
VolumePorod 25 nm3

SASDQV5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type, bound to RNA

UniProt ID: Q01560 (120-280) Serine/arginine (SR)-type shuttling mRNA binding protein NPL3

UniProt ID: None (None-None) Short RNA oligonucleotide (NPL3 binding sequence)
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3Short RNA oligonucleotide (NPL3 binding sequence) experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Short RNA oligonucleotide (NPL3 binding sequence) Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Short RNA oligonucleotide (NPL3 binding sequence) monomer, 4 kDa RNA
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 30 nm3

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1
C-terminal-binding protein 1 experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 279 nm3

SASDQX5 – C-terminal binding protein 1 mutant (CtBP1: C134Y, N138R, R141E, L150W)

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W)
C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W), 48 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 154 nm3

SASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1 (R266A, D290A, E295A, H315A)
C-terminal-binding protein 1 (R266A, D290A, E295A, H315A) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (R266A, D290A, E295A, H315A), 47 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 161 nm3

SASDQZ5 – Retinoic acid-induced protein 2 (RAI2: 303-362)

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362)
Retinoic acid-induced protein 2 (303-362) experimental SAS data
Retinoic acid-induced protein 2 (303-362) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 12 nm3

SASDQ26 – Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: L319A, S320A)
Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data
Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 10 nm3

SASDQ36 – Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

UniProt ID: Q9Y5P3 (303-362) Retinoic acid-induced protein 2 (303-362: L345A, S346A)
Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data
Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot
Sample: Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 11 nm3