SASBDB entries for UniProt ID:

SASDQE6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465: L345A, S346A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 310 nm3

SASDQF6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1+M2 mutant L319A, S320A, L345A, S346A

UniProt ID: Q13363 (1-440) C-terminal-binding protein 1

UniProt ID: Q9Y5P3 (303-465) Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A)
C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 5.1 nm
Dmax 17.5 nm
VolumePorod 387 nm3

SASDQN6 – The N-terminal domain of Phospholipase A and acyltransferase 4 (PLAAT4 NTD)

UniProt ID: Q9UL19 (1-123) Phospholipase A and acyltransferase 4
Phospholipase A and acyltransferase 4 experimental SAS data
PYMOL model
Sample: Phospholipase A and acyltransferase 4 monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 11
Crystal structure of the phospholipase A and acyltransferase 4 (PLAAT4) catalytic domain. J Struct Biol 214(4):107903 (2022)
Wehlin A, Cornaciu I, Marquez JA, Perrakis A, von Castelmur E
RgGuinier 1.9 nm
Dmax 7.5 nm
VolumePorod 31 nm3

SASDQT6 – apo-form of eukaryotic initiation factor 4A1

UniProt ID: P60842 (1-406) Eukaryotic initiation factor 4A-I
Eukaryotic initiation factor 4A-I experimental SAS data
Eukaryotic initiation factor 4A-I Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.1 nm
Dmax 9.8 nm
VolumePorod 80 nm3

SASDQU6 – eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA

UniProt ID: P60842 (1-406) Eukaryotic initiation factor 4A-I

UniProt ID: None (None-None) (AG)10-RNA
Eukaryotic initiation factor 4A-I(AG)10-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (AG)10-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
(AG)10-RNA monomer, 7 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 96 nm3

SASDQV6 – eukaryotic initiation factor 4A1 bound to 20 nt (CAA)6CA ssRNA

UniProt ID: P60842 (1-406) Eukaryotic initiation factor 4A-I

UniProt ID: None (None-None) (CAA)6CA-RNA
Eukaryotic initiation factor 4A-I(CAA)6CA-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (CAA)6CA-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
(CAA)6CA-RNA monomer, 6 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.1 nm
Dmax 12.8 nm
VolumePorod 89 nm3

SASDQW6 – multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA

UniProt ID: None (None-None) (AG)10-RNA

UniProt ID: P60842 (1-406) Eukaryotic initiation factor 4A-I
(AG)10-RNAEukaryotic initiation factor 4A-I experimental SAS data
(AG)10-RNA Eukaryotic initiation factor 4A-I Kratky plot
Sample: (AG)10-RNA monomer, 7 kDa synthetic construct RNA
Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.4 nm
Dmax 13.1 nm
VolumePorod 150 nm3

SASDQX6 – multimeric eukaryotic initiation factor 4A1 bound to 20 nt (AG)10-24bp duplex RNA

UniProt ID: P60842 (1-406) Eukaryotic initiation factor 4A-I

UniProt ID: None (None-None) (AG)10-24bp loading-RNA

UniProt ID: None (None-None) (AG)10-24bp duplex-RNA
Eukaryotic initiation factor 4A-I(AG)10-24bp loading-RNA(AG)10-24bp duplex-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein
(AG)10-24bp loading-RNA monomer, 15 kDa synthetic construct RNA
(AG)10-24bp duplex-RNA monomer, 7 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 4.9 nm
Dmax 16.2 nm
VolumePorod 230 nm3

SASDQ87 – Solution structure of colibactin ClbK

UniProt ID: Q0P7K1 (1-2154) Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK
Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK experimental SAS data
Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK Kratky plot
Sample: Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK dimer, 476 kDa Escherichia coli protein
Buffer: 50 mM Hepes pH 7.5, 200 mM NaCl, 5 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 14
Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin Structure (2023)
Bonhomme S, Contreras-Martel C, Dessen A, Macheboeuf P
RgGuinier 8.0 nm
Dmax 30.0 nm
VolumePorod 1340 nm3

SASDQ97 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E]

UniProt ID: Q06609 (1-339) DNA repair protein RAD51 homolog 1 (F86E A89E)
DNA repair protein RAD51 homolog 1 (F86E A89E) experimental SAS data
Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 3.0 nm
Dmax 14.2 nm
VolumePorod 61 nm3