SASBDB entries for UniProt ID:

SASDQD8 – Rap guanine nucleotide exchange factor Epac1 with cAMP and CE3F4 inhibitor

UniProt ID: O95398 (194-923) Rap guanine nucleotide exchange factor 3
Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 134 nm3

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain

UniProt ID: A0A1D5PXA5 (135-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
PYMOL model
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 2.3 nm
Dmax 11.5 nm
VolumePorod 41 nm3

SASDQF8 – G. gallus TRPV4 N-terminal Domain

UniProt ID: A0A1D5PXA5 (2-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 N-terminal Domain Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 3.7 nm
Dmax 19.0 nm
VolumePorod 59 nm3

SASDQG8 – G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111

UniProt ID: A0A1D5PXA5 (2-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111 Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 42 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 4.0 nm
Dmax 19.5 nm
VolumePorod 78 nm3

SASDQH8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54

UniProt ID: A0A1D5PXA5 (55-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54 Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 37 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 64 nm3

SASDQJ8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97

UniProt ID: A0A1D5PXA5 (98-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97 Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 33 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 2.5 nm
Dmax 11.9 nm
VolumePorod 41 nm3

SASDQK8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N104

UniProt ID: A0A1D5PXA5 (105-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
Transient receptor potential cation channel subfamily V member 4 Kratky plot
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 32 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 49 nm3

SASDQL8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N120

UniProt ID: A0A1D5PXA5 (121-382) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
Transient receptor potential cation channel subfamily V member 4 Kratky plot
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 29 kDa Gallus gallus protein
Buffer: 20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 2.7 nm
Dmax 12.5 nm
VolumePorod 43 nm3

SASDQM8 – G. gallus TRPV4 intrinsically disordered N-terminus

UniProt ID: A0A1D5PXA5 (2-134) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 intrinsically disordered N-terminus Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 15 kDa Gallus gallus protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 3.2 nm
Dmax 14.5 nm
VolumePorod 59 nm3

SASDQN8 – G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111

UniProt ID: A0A1D5PXA5 (2-134) Transient receptor potential cation channel subfamily V member 4
Transient receptor potential cation channel subfamily V member 4 experimental SAS data
G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111 Rg histogram
Sample: Transient receptor potential cation channel subfamily V member 4 monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16
Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U
RgGuinier 3.3 nm
Dmax 14.5 nm
VolumePorod 37 nm3