Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDQS8 – Exon1 of Pathogenic form of Huntingtin (H46) with GFP fused at the C-terminus

UniProt ID: P42858 (1-88) Huntingtin

Sample:	Huntingtin monomer, 43 kDa Homo sapiens protein
Buffer:	20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 28

The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
Elena-Real CA, Sagar A, Urbanek A, Popovic M, Morató A, Estaña A, Fournet A, Doucet C, Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N, Bernadó P

R_g^Guinier

4.2

SASDQT8 – Dark-state LOV-activated diguanylate cyclase

UniProt ID: A0A2S5LZS0 (3-314) Sensor domain-containing diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data

Sample:	Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer:	10 mM Tris, 50 mM NaCl, 2 mM MgCl2, 3% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Apr 24

LOV-activated diguanylate cyclase
Ursula Vide

R_g^Guinier	3.4	nm
D_max	15.0	nm
Volume^Porod	128	nm³

SASDQU8 – Light-state LOV-activated diguanylate cyclase

UniProt ID: A0A2S5LZS0 (3-314) Sensor domain-containing diguanylate cyclase

Sample:	Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer:	10 mM Tris, 50 mM NaCl, 2 mM MgCl2, 3% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Apr 24

LOV-activated diguanylate cyclase

R_g^Guinier	5.2	nm
D_max	18.0	nm
Volume^Porod	159	nm³

SASDQV8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-free (Apo) state

UniProt ID: A0A0B4K6R7 (434-697) Na/Ca-exchange protein, isoform D

Na/Ca-exchange protein, isoform D experimental SAS data

Na/Ca-exchange protein, isoform D Kratky plot

Sample:	Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer:	20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 2 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8

SAXS characterisation of CALX1.2 CBD12 construct
Roberto Kopke Salinas

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	48	nm³

SASDQW8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-bound state

UniProt ID: A0A0B4K6R7 (434-697) Na/Ca-exchange protein, isoform D

Sample:	Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer:	20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 50 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8

SAXS characterisation of CALX1.2 CBD12 construct

R_g^Guinier	2.7	nm
D_max	9.9	nm
Volume^Porod	32	nm³

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

R_g^Guinier

3.1

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P02791 (1-175) Ferritin light chain

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

5.8

SASDQ29 – Thyroglobulin - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P01267 (1-2769) Thyroglobulin

Sample:	Thyroglobulin monomer, 303 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

7.0

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P0DTC2 (319-566) Spike glycoprotein (ACE2 receptor binding domain)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Spike glycoprotein (ACE2 receptor binding domain) Kratky plot

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

3.0

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