SASBDB entries for UniProt ID:

SASDQ48 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 1.1 mg/mL

UniProt ID: F7X6I3 (None-None) Minimal proline dehydrogenase domain of proline utilization A (design #2)
Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 102 nm3

SASDQ58 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 2.3 mg/mL

UniProt ID: F7X6I3 (None-None) Minimal proline dehydrogenase domain of proline utilization A (design #2)
Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 102 nm3

SASDQ68 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 3.4 mg/mL

UniProt ID: F7X6I3 (None-None) Minimal proline dehydrogenase domain of proline utilization A (design #2)
Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 108 nm3

SASDQ78 – YbiB-ObgE GTPase complex

UniProt ID: P30177 (1-320) Uncharacterized protein YbiB

UniProt ID: B1XHF9 (1-390) GTPase Obg
Uncharacterized protein YbiBGTPase Obg experimental SAS data
Uncharacterized protein YbiB GTPase Obg Kratky plot
Sample: Uncharacterized protein YbiB dimer, 75 kDa Escherichia coli (strain … protein
GTPase Obg dimer, 91 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 24
YbiB: a novel interactor of the GTPase ObgE. Nucleic Acids Res (2023)
Deckers B, Vercauteren S, De Kock V, Martin C, Lazar T, Herpels P, Dewachter L, Verstraeten N, Peeters E, Ballet S, Michiels J, Galicia C, Versées W
RgGuinier 4.7 nm
Dmax 18.6 nm
VolumePorod 291 nm3

SASDQ88 – recombinant p123 expressed protein (Gli123)

UniProt ID: F8WJV6 (19-1128) p123 expressed protein
p123 expressed protein experimental SAS data
DAMMIN model
Sample: P123 expressed protein dimer, 248 kDa Mycoplasma mobile (strain … protein
Buffer: 100 mM ammonium acetate, pH: 6.8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Oct 25
Structure and Function of Gli123 Involved in Mycoplasma mobile Gliding. J Bacteriol :e0034022 (2023)
Matsuike D, Tahara YO, Nonaka T, Wu HN, Hamaguchi T, Kudo H, Hayashi Y, Arai M, Miyata M
RgGuinier 8.2 nm
Dmax 31.6 nm
VolumePorod 436 nm3

SASDQ98 – Full-length human DPCD

UniProt ID: Q9BVM2 (1-203) Protein DPCD
Protein DPCD experimental SAS data
Full-length human DPCD Rg histogram
Sample: Protein DPCD monomer, 23 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2018 Nov 8
DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling. Cell Rep 43(2):113713 (2024)
Mao YQ, Seraphim TV, Wan Y, Wu R, Coyaud E, Bin Munim M, Mollica A, Laurent E, Babu M, Mennella V, Raught B, Houry WA
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 28 nm3

SASDQA8 – Rap guanine nucleotide exchange factor Epac1

UniProt ID: O95398 (194-923) Rap guanine nucleotide exchange factor 3
Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 130 nm3

SASDQB8 – Rap guanine nucleotide exchange factor Epac1 with cAMP

UniProt ID: O95398 (194-923) Rap guanine nucleotide exchange factor 3
Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 140 nm3

SASDQC8 – Rap guanine nucleotide exchange factor Epac1 with CE3F4 inhibitor

UniProt ID: O95398 (194-923) Rap guanine nucleotide exchange factor 3
Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.3 nm
Dmax 11.4 nm
VolumePorod 140 nm3

SASDQD8 – Rap guanine nucleotide exchange factor Epac1 with cAMP and CE3F4 inhibitor

UniProt ID: O95398 (194-923) Rap guanine nucleotide exchange factor 3
Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 134 nm3