SASBDB entries for UniProt ID:

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.0 nm
Dmax 27.0 nm
VolumePorod 241 nm3

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 5.5 nm
Dmax 26.0 nm
VolumePorod 300 nm3

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 6.9 nm
Dmax 31.5 nm
VolumePorod 490 nm3

SASDRQ3 – Trypsin + Aprotinin: Time-Resolved 10 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 23 nm3

SASDRR3 – Trypsin + Aprotinin: Time-Resolved 30 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDRS3 – Trypsin + Aprotinin: Time-Resolved 100 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 30 nm3

SASDRT3 – Trypsin + Aprotinin: Time-Resolved 400 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 27 nm3

SASDRU3 – Trypsin + Aprotinin: Time-Resolved 630 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 25 nm3

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 1.8 nm
Dmax 5.3 nm
VolumePorod 28 nm3

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

UniProt ID: P00760 (1-246) Serine protease 1

UniProt ID: P00974 (1-100) Pancreatic trypsin inhibitor
Serine protease 1Pancreatic trypsin inhibitor experimental SAS data
Serine protease 1 Pancreatic trypsin inhibitor Kratky plot
Sample: Serine protease 1 monomer, 26 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer: 20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.0 nm
Dmax 5.3 nm
VolumePorod 29 nm3