SASBDB entries for UniProt ID:

SASDR46 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax9

UniProt ID: Q43715 (163-440) Protein TOC75, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment ax9
Protein TOC75, chloroplasticsynthetic antigen binding fragment ax9 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax9 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax9 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 123 nm3

SASDR56 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax14

UniProt ID: Q43715 (163-440) Protein TOC75, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment ax14
Protein TOC75, chloroplasticsynthetic antigen binding fragment ax14 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax14 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax14 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 4.1 nm
Dmax 15.5 nm
VolumePorod 150 nm3

SASDR66 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax15

UniProt ID: Q43715 (163-440) Protein TOC75, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment ax15
Protein TOC75, chloroplasticsynthetic antigen binding fragment ax15 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax15 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax15 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 123 nm3

SASDR76 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax17

UniProt ID: Q43715 (163-440) Protein TOC75, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment ax17
Protein TOC75, chloroplasticsynthetic antigen binding fragment ax17 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax17 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax17 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 118 nm3

SASDR86 – Human Survivin (BIRC5)

UniProt ID: O15392 (1-142) Baculoviral IAP repeat-containing protein 5
Baculoviral IAP repeat-containing protein 5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Baculoviral IAP repeat-containing protein 5 dimer, 34 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 10
Survivin prevents the Polycomb Repressor Complex 2 from methylating Histone 3 lysine 27 iScience :106976 (2023)
Jensen M, Chandrasekaran V, García-Bonete M, Li S, Anindya A, Andersson K, Erlandsson M, Oparina N, Burmann B, Brath U, Panchenko A, Maria Bokarewa I, Katona G
RgGuinier 2.9 nm
Dmax 10.4 nm
VolumePorod 53 nm3

SASDR96 – Human DjC20/DnaJC20/HscB iron-sulfur cluster co-chaperone protein

UniProt ID: Q8IWL3 (28-235) Iron-sulfur cluster co-chaperone protein HscB
Iron-sulfur cluster co-chaperone protein HscB experimental SAS data
DAMMIF model
Sample: Iron-sulfur cluster co-chaperone protein HscB monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 50 mM NaCl, 5 mM KCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 Aug 14
Structural characterization of the human DjC20/HscB cochaperone in solution Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140970 (2023)
de Souza Coto A, Pereira A, Oliveira S, de Oliveira Moritz M, da Rocha A, Dores-Silva P, da Silva N, de Araújo Nogueira A, Gava L, Seraphim T, Borges J
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 40 nm3

SASDRA6 – Cell division protein FtsQ N-terminal soluble domain

UniProt ID: P9WNA1 (1-99) Cell division protein FtsQ
Cell division protein FtsQ experimental SAS data
Cell division protein FtsQ Kratky plot
Sample: Cell division protein FtsQ monomer, 11 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM phosphate buffer, 25 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 5-ID-D DND-CAT, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jun 28
An Arg/Ala-rich helix in the N-terminal region of M. tuberculosis FtsQ is a potential membrane anchor of the Z-ring. Commun Biol 6(1):311 (2023)
Smrt ST, Escobar CA, Dey S, Cross TA, Zhou HX
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 21 nm3

SASDRC6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with ADP-AlF4

UniProt ID: Q16539 (10-360) Mitogen-activated protein kinase 14

UniProt ID: None (None-None) Dual specificity mitogen-activated protein kinase kinase 6

UniProt ID: None (None-None) Adenosine 5'-Diphosphate Tetrafluoroaluminate
Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 6Adenosine 5'-Diphosphate Tetrafluoroaluminate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
Adenosine 5'-Diphosphate Tetrafluoroaluminate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 3.2 nm
Dmax 12.1 nm
VolumePorod 113 nm3

SASDRD6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with AMP-PCP

UniProt ID: Q16539 (10-360) Mitogen-activated protein kinase 14

UniProt ID: None (None-None) Dual specificity mitogen-activated protein kinase kinase 6

UniProt ID: None (None-None) 5′‐adenylyl methylenediphosphonate
Mitogen-activated protein kinase 14Dual specificity mitogen-activated protein kinase kinase 65′‐adenylyl methylenediphosphonate experimental SAS data
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 5′‐adenylyl methylenediphosphonate Kratky plot
Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein
Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein
5′‐adenylyl methylenediphosphonate monomer, 1 kDa
Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15
Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation Science 381(6663):1217-1225 (2023)
Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 116 nm3

SASDRV6 – Transcription regulator PaaR2

UniProt ID: Q8XAD6 (1-123) Phage repressor protein CI (C120S)
Phage repressor protein CI (C120S) experimental SAS data
OTHER model
Sample: Phage repressor protein CI (C120S) octamer, 122 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 16
Regulation of the Escherichia coli paaR2-paaA2-ParD2 toxin-antitoxin system
Marusa Prolic Kalinsek
RgGuinier 5.0 nm
Dmax 16.8 nm
VolumePorod 428 nm3