SASBDB entries for UniProt ID:

SASDS42 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 41% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Aug 24
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 11.1 nm
Dmax 32.4 nm
VolumePorod 138 nm3

SASDS52 – Sulfite reductase crosslinked dodecamer

UniProt ID: P17846 (None-None) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 10.3 nm
Dmax 31.0 nm
VolumePorod 1170 nm3

SASDS62 – Sulfite reductase flavoprotein truncated linker (ΔAAPSQS) octamer

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 526 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 25
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 13.7 nm
Dmax 25.5 nm
VolumePorod 961 nm3

SASDS72 – Sulfite reductase truncated linker (ΔAAPSQS) dodecamer

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 528 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 26
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 10.8 nm
Dmax 30.3 nm
VolumePorod 1260 nm3

SASDS82 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 0% D2O

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)Sulfite reductase [NADPH] hemoprotein beta-component experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Sulfite reductase [NADPH] hemoprotein beta-component Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 2
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 9.5 nm
Dmax 28.3 nm
VolumePorod 1010 nm3

SASDS92 – Reconstituted sulfite reductase crosslinked dodecamer with partially deuterated hemoprotein in 100% D2O

UniProt ID: P17846 (1-570) Sulfite reductase [NADPH] hemoprotein beta-component

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Sulfite reductase [NADPH] hemoprotein beta-componentSulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sulfite reductase [NADPH] hemoprotein beta-component Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Kratky plot
Sample: Sulfite reductase [NADPH] hemoprotein beta-component tetramer, 256 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jun 27
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 8.2 nm
Dmax 25.1 nm
VolumePorod 839 nm3

SASDSA2 – Sulfite reductase flavoprotein reduced with 10 molar equivalents sodium dithionite

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component
Sulfite reductase [NADPH] flavoprotein alpha-component experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component octamer, 530 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 7.9 nm
Dmax 27.6 nm
VolumePorod 1060 nm3

SASDSB2 – Sulfite reductase flavoprotein crosslinked reduced with 10 molar equivalents sodium dithionite

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C)
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (E121C, C162T, C552S, N556C) octamer, 566 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 8.0 nm
Dmax 25.3 nm
VolumePorod 827 nm3

SASDSC2 – Sulfite reductase truncated linker (ΔAAPSQS) flavoprotein reduced with 10 molar equivalents sodium dithionite

UniProt ID: P38038 (1-599) Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217)
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 526 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 952 nm3

SASDSD2 – Recombinant Heavy-chain-only nanobody C5-Fc (glycosylated)

UniProt ID: None (None-None) Immunoglobulin heavy constant gamma 1
Immunoglobulin heavy constant gamma 1 experimental SAS data
PYMOL model
Sample: Immunoglobulin heavy constant gamma 1 monomer, 78 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Sep 28
The solution structure of the heavy chain-only C5-Fc nanobody reveals exposed variable regions that are optimal for COVID-19 antigen interactions. J Biol Chem :105337 (2023)
Gao X, Thrush JW, Gor J, Naismith JH, Owens RJ, Perkins SJ
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 157 nm3