Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDSA6 – Human Ubiquilin-2 (UBQLN2)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2

Sample:	Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.2	nm
D_max	22.5	nm
Volume^Porod	330	nm³

SASDSB6 – Human Ubiquilin-4 (UBQLN4)

UniProt ID: Q9NRR5 (1-601) Ubiquilin-4

Sample:	Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.1	nm
D_max	21.7	nm
Volume^Porod	333	nm³

SASDSC6 – Human Ubiquilin-2, ∆STI1-1 domain deletion (UBQLN2∆STI1-1)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (∆170-260)

Ubiquilin-2 (∆170-260) experimental SAS data

Sample:	Ubiquilin-2 (∆170-260) dimer, 110 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.4	nm
D_max	26.0	nm
Volume^Porod	357	nm³

SASDSD6 – Human Ubiquilin-2 with ∆UBL and ∆STI1-1 domain deletions (UBQLN2∆STI1-1∆UBL)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (∆33-103, ∆170-260)

Ubiquilin-2 (∆33-103, ∆170-260) experimental SAS data

Ubiquilin-2 (∆33-103, ∆170-260) Kratky plot

Sample:	Ubiquilin-2 (∆33-103, ∆170-260) dimer, 94 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.6	nm
D_max	24.5	nm
Volume^Porod	326	nm³

SASDSE6 – Human Ubiquilin-2, ∆1-108 N-terminal deletion (UBQLN2 109-624)

UniProt ID: Q9UHD9 (109-624) Ubiquilin-2 (∆1-108)

Ubiquilin-2 (∆1-108) experimental SAS data

Sample:	Ubiquilin-2 (∆1-108) dimer, 108 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.2	nm
D_max	23.3	nm
Volume^Porod	281	nm³

SASDSF6 – Human Ubiquilin-2, C-terminal construct (UBQLN2 450-624W)

UniProt ID: Q9UHD9 (450-624) Ubiquilin-2 (∆1-449)-W

Ubiquilin-2 (∆1-449)-W experimental SAS data

Sample:	Ubiquilin-2 (∆1-449)-W dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	3.8	nm
D_max	18.5	nm
Volume^Porod	50	nm³

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (∆379-462)

Ubiquilin-2 (∆379-462) experimental SAS data

Sample:	Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.4	nm
D_max	22.4	nm
Volume^Porod	121	nm³

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (∆487-538)

Ubiquilin-2 (∆487-538) experimental SAS data

Sample:	Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation.
Sarasi Galagedera

R_g^Guinier	4.8	nm
D_max	19.5	nm
Volume^Porod	288	nm³

SASDSJ6 – Human Ubiquilin-2, P497E phosphomimetic point mutant (UBQLN2 P497E)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (P497E)

Ubiquilin-2 (P497E) experimental SAS data

Sample:	Ubiquilin-2 (P497E) dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	5.3	nm
D_max	21.3	nm
Volume^Porod	340	nm³

SASDSK6 – Human Ubiquilin-2, P497L ALS-linked point mutant (UBQLN2 P497L)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (P497L)

Ubiquilin-2 (P497L) experimental SAS data

Sample:	Ubiquilin-2 (P497L) dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs
Sarasi Galagedera

R_g^Guinier	7.3	nm
D_max	28.8	nm
Volume^Porod	1365	nm³

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