SASBDB entries for UniProt ID:

SASDSS5 – SARS-CoV-2 Main Protease H163A Mutant - 3.0 mg/mL

UniProt ID: P0DTD1 (3264-3569) Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant)
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 102 nm3

SASDST5 – SARS-CoV-2 Main Protease H163A Mutant - 6.3 mg/mL

UniProt ID: P0DTD1 (3264-3569) Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant)
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 7.7 nm
VolumePorod 98 nm3

SASDSU5 – Histidine-phosphotransferase from Candida albicans

UniProt ID: Q59WC6 (12-184) Phosphorelay intermediate protein YPD1
Phosphorelay intermediate protein YPD1 experimental SAS data
SASREF model
Sample: Phosphorelay intermediate protein YPD1 monomer, 19 kDa Candida albicans (strain … protein
Buffer: 50 mM Tris-HCl pH 8, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Oct 10
Structural and functional insights underlying recognition of histidine phosphotransfer protein in fungal phosphorelay systems Communications Biology 7(1) (2024)
Paredes-Martínez F, Eixerés L, Zamora-Caballero S, Casino P
RgGuinier 2.5 nm
Dmax 11.5 nm
VolumePorod 33 nm3

SASDSV5 – Dimeric structure of Zn-TtPetA (Ubiquinol-cytochrome c reductase)

UniProt ID: D1MZ11 (49-197) Ubiquinol-cytochrome c reductase iron-sulfur subunit
Ubiquinol-cytochrome c reductase iron-sulfur subunit experimental SAS data
OTHER model
Sample: Ubiquinol-cytochrome c reductase iron-sulfur subunit dimer, 33 kDa Thermochromatium tepidum protein
Buffer: 10 mM Tris-HCl pH 7.6, 150 mM NaCl, 5% glycerol, pH: 7.6
Experiment: SAXS data collected at BL38B1, SPring-8 on 2023 Apr 26
Structure of a putative immature form of a Rieske-type iron-sulfur protein in complex with zinc chloride. Commun Chem 6(1):190 (2023)
Tsutsumi E, Niwa S, Takeda R, Sakamoto N, Okatsu K, Fukai S, Ago H, Nagao S, Sekiguchi H, Takeda K
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 38 nm3

SASDSW5 – B cell lymphoma 7 protein family member A (BCL7A)

UniProt ID: Q4VC05-2 (1-231) Isoform 2 of B-cell CLL/lymphoma 7 protein family member A
Isoform 2 of B-cell CLL/lymphoma 7 protein family member A experimental SAS data
Isoform 2 of B-cell CLL/lymphoma 7 protein family member A Kratky plot
Sample: Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.8 nm
Dmax 28.8 nm

SASDSX5 – Nucleosome core particle (NCP) composed of the Widom 601 DNA

UniProt ID: P84233 (1-135) Histone H3.2

UniProt ID: P62799 (1-103) Histone H4

UniProt ID: Q6AZJ8 (1-130) Histone H2A

UniProt ID: P02281 (1-126) Histone H2B 1.1

UniProt ID: None (None-None) Widom 601 DNA sequence
Histone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.3 nm
Dmax 12.3 nm
VolumePorod 350 nm3

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)

UniProt ID: Q4VC05-2 (1-231) Isoform 2 of B-cell CLL/lymphoma 7 protein family member A

UniProt ID: P84233 (1-135) Histone H3.2

UniProt ID: P62799 (1-103) Histone H4

UniProt ID: Q6AZJ8 (1-130) Histone H2A

UniProt ID: P02281 (1-126) Histone H2B 1.1

UniProt ID: None (None-None) Widom 601 DNA sequence
Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data
EOM/RANCH model
Sample: Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein
Histone H3.2 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 23 kDa Xenopus laevis protein
Histone H2A dimer, 28 kDa Xenopus laevis protein
Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein
Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer: 20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 2
BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani
RgGuinier 4.7 nm
Dmax 24.4 nm
VolumePorod 400 nm3

SASDS36 – Human RAD51C-XRCC3 at pH 8 in the presence of ATP and vanadate, a phosphate mimic

UniProt ID: O43502-1 (10-367) Isoform 1 of DNA repair protein RAD51 homolog 3

UniProt ID: O43542 (1-346) DNA repair protein XRCC3
Isoform 1 of DNA repair protein RAD51 homolog 3DNA repair protein XRCC3 experimental SAS data
ITASSER model
Sample: Isoform 1 of DNA repair protein RAD51 homolog 3 monomer, 40 kDa Homo sapiens protein
DNA repair protein XRCC3 monomer, 38 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 8, 100 mM NaCl, 2.5 mM ATP, 2.5 mM MgCl2, and 0.1 mM Na3VO4, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 May 5
RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles Nature Communications 14(1) (2023)
Longo M, Roy S, Chen Y, Tomaszowski K, Arvai A, Pepper J, Boisvert R, Kunnimalaiyaan S, Keshvani C, Schild D, Bacolla A, Williams G, Tainer J, Schlacher K
RgGuinier 3.6 nm
Dmax 14.0 nm
VolumePorod 132 nm3

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

UniProt ID: F5S9L7 (468-708) RTX toxin transporter
RTX toxin transporter experimental SAS data
GASBOR model
Sample: RTX toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer: 100 mM HEPES, 10 % glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 48 nm3

SASDS76 – Antitoxin HigA-2

UniProt ID: Q9KMA5 (2-104) Antitoxin HigA-2
Antitoxin HigA-2 experimental SAS data
Antitoxin HigA-2 Kratky plot
Sample: Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Apr 6
Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
Hadži S, Živič Z, Kovačič M, Zavrtanik U, Haeserts S, Charlier D, Plavec J, Volkov AN, Lah J, Loris R
RgGuinier 2.3 nm
Dmax 9.6 nm
VolumePorod 43 nm3