SASBDB entries for UniProt ID:

SASDSL6 – Human Ubiquilin-2, R12G R15D Q21E triple point mutant (UBQLN2 GDE)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (R12G, R15D, Q21E)
Ubiquilin-2 (R12G, R15D, Q21E) experimental SAS data
Ubiquilin-2 (R12G, R15D, Q21E) Kratky plot
Sample: Ubiquilin-2 (R12G, R15D, Q21E) dimer, 131 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
UBQLN2 domain deletion constructs
Sarasi Galagedera
RgGuinier 5.1 nm
Dmax 21.3 nm
VolumePorod 330 nm3

SASDSM6 – Human Ubiquilin-2, ∆551-569 deletion construct (UBQLN2∆551-569)

UniProt ID: Q9UHD9 (1-624) Ubiquilin-2 (∆551-569)
Ubiquilin-2 (∆551-569) experimental SAS data
Ubiquilin-2 (∆551-569) Kratky plot
Sample: Ubiquilin-2 (∆551-569) dimer, 127 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
UBQLN2 domain deletion constructs
Sarasi Galagedera
RgGuinier 5.2 nm
Dmax 23.4 nm
VolumePorod 332 nm3

SASDSN6 – Human full length RAD23B

UniProt ID: P54727 (1-409) UV excision repair protein RAD23 homolog B
UV excision repair protein RAD23 homolog B experimental SAS data
UV excision repair protein RAD23 homolog B Kratky plot
Sample: UV excision repair protein RAD23 homolog B monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.6 nm
Dmax 26.0 nm
VolumePorod 103 nm3

SASDSP6 – Human RAD23B, ∆UBL domain deletion (RAD23B∆UBL)

UniProt ID: P54727 (80-409) UV excision repair protein RAD23 homolog B (∆1-79)
UV excision repair protein RAD23 homolog B (∆1-79) experimental SAS data
UV excision repair protein RAD23 homolog B (∆1-79) Kratky plot
Sample: UV excision repair protein RAD23 homolog B (∆1-79) monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.6 nm
Dmax 21.6 nm
VolumePorod 92 nm3

SASDSQ6 – Human RAD23B, ∆UBA-1 domain deletion (RAD23B∆UBA1)

UniProt ID: P54727 (1-409) UV excision repair protein RAD23 homolog B (∆186-231)
UV excision repair protein RAD23 homolog B (∆186-231) experimental SAS data
UV excision repair protein RAD23 homolog B (∆186-231) Kratky plot
Sample: UV excision repair protein RAD23 homolog B (∆186-231) monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.6 nm
Dmax 21.7 nm
VolumePorod 95 nm3

SASDSR6 – Human RAD23B, ∆UBA-2 domain deletion (RAD23B∆UBA2)

UniProt ID: P54727 (1-361) UV excision repair protein RAD23 homolog B (∆362-409)
UV excision repair protein RAD23 homolog B (∆362-409) experimental SAS data
UV excision repair protein RAD23 homolog B (∆362-409) Kratky plot
Sample: UV excision repair protein RAD23 homolog B (∆362-409) monomer, 38 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 79 nm3

SASDSS6 – Human RAD23B, ∆STI1 domain deletion (RAD23B∆STI1)

UniProt ID: P54727 (1-409) UV excision repair protein RAD23 homolog B (∆274-306)
UV excision repair protein RAD23 homolog B (∆274-306) experimental SAS data
UV excision repair protein RAD23 homolog B (∆274-306) Kratky plot
Sample: UV excision repair protein RAD23 homolog B (∆274-306) monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.6 nm
Dmax 24.2 nm
VolumePorod 103 nm3

SASDST6 – Human RAD23B with ∆UBA-1 and ∆UBA-2 domain deletions (RAD23B∆UBA1∆UBA2)

UniProt ID: P54727 (1-409) UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409)
UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) experimental SAS data
UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) Kratky plot
Sample: UV excision repair protein RAD23 homolog B (∆186-231, ∆362-409) monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 22
Domain deletion constructs of hHR23B
Sarasi Galagedera
RgGuinier 4.9 nm
Dmax 25.5 nm
VolumePorod 100 nm3

SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

UniProt ID: O67633 (1-183) DUF507 family protein
DUF507 family protein experimental SAS data
CUSTOM IN-HOUSE model
Sample: DUF507 family protein monomer, 22 kDa Aquifex aeolicus (strain … protein
Buffer: 20 mM Hepes pH 7.2, 400 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 7
Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold. Sci Rep 13(1):13496 (2023)
McKay CE, Cheng J, Tanner JJ
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 39 nm3

SASDSY6 – Outer membrane protein MIP (LpMIP) apo state

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP
Outer membrane protein MIP experimental SAS data
SREFLEX model
Sample: Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16
Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 66 nm3