SASBDB entries for UniProt ID:

SASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)

UniProt ID: Q9Z7M7 (67-382) Uncharacterized protein

UniProt ID: O08816 (141-273) Actin nucleation-promoting factor WASL
Uncharacterized proteinActin nucleation-promoting factor WASL experimental SAS data
SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL) Rg histogram
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 102 nm3

SASDTV5 – Human full-length Annexin A11 (apo form)

UniProt ID: P50995 (2-505) Annexin A11
Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 5 mM EDTA, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
Dudas E, Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 15.9 nm
VolumePorod 84 nm3

SASDTW5 – Human full-length Annexin A11 (holo form, with calcium)

UniProt ID: P50995 (2-505) Annexin A11
Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 500 µM CaCl2, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
Dudas E, Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 17.1 nm
VolumePorod 83 nm3

SASDT76 – Neurofilament low intrinsically disordered tail domain aggregate - 2.68 mg/ml - 20 mM Tris pH 8.0

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 26.4 nm

SASDT86 – Neurofilament low intrinsically disordered tail domain aggregate - 1.80 mg/ml - 20 mM Tris pH 8.0

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 21.9 nm

SASDT96 – Neurofilament low intrinsically disordered tail domain aggregate - 1.00 mg/ml - 20 mM Tris pH 8.0

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 22.9 nm

SASDTA6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.50 mg/ml - 20 mM Tris pH 8.0

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 22.6 nm

SASDTB6 – Neurofilament low intrinsically disordered tail domain aggregate - 1.3 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 17.8 nm

SASDTC6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.73 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 19.4 nm

SASDTD6 – Neurofilament low intrinsically disordered tail domain aggregate - 0.57 mg/ml - 20 mM Tris pH 8.0, 150 mM NaCl

UniProt ID: P08551 (399-553) Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553)
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.00, 150 mM NaCl, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 22.2 nm