SASBDB entries for UniProt ID:

SASDTN4 – Tissue Transglutaminase R580K mutant + 100 µM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2 (R580K)
Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K), 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.9 nm
Dmax 27.0 nm
VolumePorod 381 nm3

SASDTP4 – Tissue Transglutaminase R580K mutant + 1 mM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2 (R580K)
Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K), 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.7 nm
Dmax 30.0 nm
VolumePorod 283 nm3

SASDTQ4 – Tissue Transglutaminase R580K mutant + 2 mM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2 (R580K)
Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K), 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.0 nm
VolumePorod 264 nm3

SASDTR4 – Tissue Transglutaminase R580K mutant + 5 mM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2 (R580K)
Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K), 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDTS4 – Type 2 DNA topoisomerase 6 subunit B-like

UniProt ID: J3QMY9 (1-554) Type 2 DNA topoisomerase 6 subunit B-like
Type 2 DNA topoisomerase 6 subunit B-like experimental SAS data
Type 2 DNA topoisomerase 6 subunit B-like Rg histogram
Sample: Type 2 DNA topoisomerase 6 subunit B-like monomer, 61 kDa Mus musculus protein
Buffer: 20 mM HEPES, 500 mM KCl, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 12
The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization Nucleic Acids Research (2024)
Diagouraga B, Tambones I, Carivenc C, Bechara C, Nadal M, de Massy B, le Maire A, Robert T
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 114 nm3

SASDTT4 – SnoaL-like domain-containing protein apoform (SLDCP concentrated monomeric SEC peak)

UniProt ID: D1C7H4 (1-108) SnoaL-like domain-containing protein
SnoaL-like domain-containing protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: SnoaL-like domain-containing protein monomer, 12 kDa Sphaerobacter thermophilus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 6
Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes. Biochim Biophys Acta Bioenerg 1865(3):149043 (2024)
Likkei K, Moldenhauer M, Tavraz NN, Egorkin NA, Slonimskiy YB, Maksimov EG, Sluchanko NN, Friedrich T
RgGuinier 1.5 nm
Dmax 5.0 nm
VolumePorod 18 nm3

SASDTU4 – 24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

UniProt ID: Q9ZLI1 (5-167) Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion)
Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
CORAL model
Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) 24-mer, 755 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 30
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2010 nm3

SASDTV4 – 8×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

UniProt ID: Q9ZLI1 (5-167) Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion)
Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
PHYRE2 model
Sample: Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) octamer, 252 kDa Helicobacter pylori (strain … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 17
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 5.4 nm
Dmax 16.0 nm
VolumePorod 618 nm3

SASDTW4 – Ferritin from Helicobacter pylori with an N-terminal His6-tag

UniProt ID: Q9ZLI1 (5-167) Bacterial non-heme ferritin (N19Q, I59V)
Bacterial non-heme ferritin (N19Q, I59V) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bacterial non-heme ferritin (N19Q, I59V) 24-mer, 480 kDa Helicobacter pylori (strain … protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Dec 14
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
Sudarev V, Gette M, Bazhenov S, Tilinova O, Zinovev E, Manukhov I, Kuklin A, Ryzhykau Y, Vlasov A
RgGuinier 5.6 nm
Dmax 14.5 nm
VolumePorod 674 nm3

SASDTX4 – G-actin - Skeletal muscle actin from Oryctolagus cuniculus: globular actin monomers

UniProt ID: P68135 (1-377) Actin, alpha skeletal muscle
Actin, alpha skeletal muscle experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer: 5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1
Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications :149340 (2023)
Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I
RgGuinier 2.9 nm
Dmax 8.5 nm
VolumePorod 58 nm3