SASBDB entries for UniProt ID:

SASDTD5 – N-terminally truncated E3 ubiquitin-protein ligase HACE1

UniProt ID: Q8IYU2 (22-909) E3 ubiquitin-protein ligase HACE1
E3 ubiquitin-protein ligase HACE1 experimental SAS data
MULTIFOXS model
Sample: E3 ubiquitin-protein ligase HACE1 monomer, 100 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 50 mM NaCl, 5 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 21
Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1 Nature Structural & Molecular Biology (2024)
Düring J, Wolter M, Toplak J, Torres C, Dybkov O, Fokkens T, Bohnsack K, Urlaub H, Steinchen W, Dienemann C, Lorenz S
RgGuinier 4.6 nm
Dmax 17.8 nm
VolumePorod 219 nm3

SASDTK5 – Heme acquisition system protein A (39.9 kDa dimer from Pseudomonas protegens PF-5)

UniProt ID: Q4K5N8 (1-183) Heme acquisition protein HasAp
Heme acquisition protein HasAp experimental SAS data
DAMMIN model
Sample: Heme acquisition protein HasAp dimer, 38 kDa Pseudomonas fluorescens (strain … protein
Buffer: 50 mM CHES, 5 % glycerol, pH: 9.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Feb 24
Heme-substituted protein assembly bridged by synthetic porphyrin: achieving controlled configuration while maintaining rotational freedom RSC Advances 14(13):8829-8836 (2024)
Inaba H, Shisaka Y, Ariyasu S, Sakakibara E, Ueda G, Aiba Y, Shimizu N, Sugimoto H, Shoji O
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 54 nm3

SASDTL5 – Conjugal transfer accessory disulfide isomerase protein TrbB from the F plasmid (GST-fusion)

UniProt ID: P18035 (1-181) Protein TrbB (GST-fusion)
Protein TrbB (GST-fusion) experimental SAS data
DAMMIF model
Sample: Protein TrbB (GST-fusion) dimer, 93 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 0.05% NP40, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23
Structural insights into the disulfide isomerase and chaperone activity of TrbB of the F plasmid type IV secretion system Current Research in Structural Biology 8:100156 (2024)
Apostol A, Bragagnolo N, Rodriguez C, Audette G
RgGuinier 4.0 nm
Dmax 18.0 nm
VolumePorod 153 nm3

SASDTM5 – Proteusin peptide

UniProt ID: A0A1Z4BZH1 (1-81) NHLP leader peptide family natural product (I56V)
NHLP leader peptide family natural product (I56V) experimental SAS data
NHLP leader peptide family natural product (I56V) Kratky plot
Sample: NHLP leader peptide family natural product (I56V) monomer, 9 kDa Methylovulum psychrotolerans protein
Buffer: 20 mM sodium phosphate (pH 7.5), 100 mM NaCl and 48 µM FAD, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jul 28
Disordered regions in proteusin peptides guide post-translational modification by a flavin-dependent RiPP brominase. Nat Commun 15(1):1265 (2024)
Nguyen NA, Vidya FNU, Yennawar NH, Wu H, McShan AC, Agarwal V
RgGuinier 1.9 nm
Dmax 5.5 nm
VolumePorod 39 nm3

SASDTN5 – The D2 domain of E3 ubiquitin-protein ligase DTX3L (I101-Q200)

UniProt ID: Q8TDB6 (101-200) E3 ubiquitin-protein ligase DTX3L
E3 ubiquitin-protein ligase DTX3L experimental SAS data
E3 ubiquitin-protein ligase DTX3L Kratky plot
Sample: E3 ubiquitin-protein ligase DTX3L tetramer, 46 kDa Homo sapiens protein
Buffer: 30 mM HEPES, 350 mM NaCl, 5% (v/v) glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Dec 6
Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor. bioRxiv (2023)
Vela-Rodríguez C, Yang C, Alanen HI, Eki R, Abbas TA, Maksimainen MM, Glumoff T, Duman R, Wagner A, Paschal BM, Lehtiö L
RgGuinier 2.9 nm
Dmax 112.0 nm
VolumePorod 91 nm3

SASDTP5 – Invariant surface glycoprotein 75 (N134A) amino acids 29-468

UniProt ID: Q26769 (29-468) Invariant surface glycoprotein (N134A)
Invariant surface glycoprotein (N134A) experimental SAS data
CORAL model
Sample: Invariant surface glycoprotein (N134A) monomer, 49 kDa Trypanosoma brucei protein
Buffer: 20 mM Tris-HCl, 75 mM KCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 18
Trypanosoma brucei Invariant Surface Glycoprotein 75 Is an Immunoglobulin Fc Receptor Inhibiting Complement Activation and Antibody-Mediated Cellular Phagocytosis. J Immunol (2024)
Mikkelsen JH, Stødkilde K, Jensen MP, Hansen AG, Wu Q, Lorentzen J, Graversen JH, Andersen GR, Fenton RA, Etzerodt A, Thiel S, Andersen CBF
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 127 nm3

SASDTQ5 – SemDΔAPH (apo form)

UniProt ID: Q9Z7M7 (67-382) Uncharacterized protein
Uncharacterized protein experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 67 nm3

SASDTR5 – BR-GBD (apo form of actin nucleation-promoting factor WASL)

UniProt ID: O08816 (141-273) Actin nucleation-promoting factor WASL
Actin nucleation-promoting factor WASL experimental SAS data
Actin nucleation-promoting factor WASL Kratky plot
Sample: Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.0 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDTS5 – Sorting nexin-9, N-terminus - SNX9-SH3 (apo form)

UniProt ID: Q9Y5X1 (1-160) Sorting nexin-9
Sorting nexin-9 experimental SAS data
Sorting nexin-9 Kratky plot
Sample: Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 42 nm3

SASDTT5 – SemDΔAPH bound to Sorting nexin-9, N-terminus - SNX9-SH3

UniProt ID: Q9Z7M7 (67-382) Uncharacterized protein

UniProt ID: Q9Y5X1 (1-160) Sorting nexin-9
Uncharacterized proteinSorting nexin-9 experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 94 nm3