SASBDB entries for UniProt ID:

SASDUQ3 – C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV)

UniProt ID: P0C1C6 (405-448) Protein W
Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV) Rg histogram
Sample: Protein W monomer, 6 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 8 nm3

SASDUR3 – C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV)

UniProt ID: P0C1C7 (407-450) Protein W
Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV) Rg histogram
Sample: Protein W monomer, 6 kDa Henipavirus nipahense protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 8 nm3

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

UniProt ID: A0A349B205 (1-608) GGDEF domain-containing protein
GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – Light-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

UniProt ID: A0A349B205 (1-608) GGDEF domain-containing protein
GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3

SASDUV3 – SAXS analysis of the LrpA backbone-Pilin Subunit from Ligilactobacillus ruminis

UniProt ID: E7FRT5 (35-472) LPXTG-motif cell wall anchor domain protein
LPXTG-motif cell wall anchor domain protein experimental SAS data
ALPHAFOLD model
Sample: LPXTG-motif cell wall anchor domain protein monomer, 49 kDa Ligilactobacillus ruminis ATCC … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 1
The crystal structure of the N-terminal domain of the backbone pilin LrpA reveals a new closure-and-twist motion for assembling dynamic pili in Ligilactobacillus ruminis Acta Crystallographica Section D Structural Biology 80(7):474-492 (2024)
Prajapati A, Palva A, von Ossowski I, Krishnan V
RgGuinier 3.5 nm
Dmax 10.6 nm
VolumePorod 59 nm3

SASDUW3 – Calcium activated full-length gelsolin

UniProt ID: P06396 (25-782) Gelsolin
Gelsolin experimental SAS data
PYMOL model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, pH8, 45 mM NaCl, 1 mM EGTA + 4 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 129 nm3

SASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
Actin, cytoplasmic 1 experimental SAS data
PYMOL model
Sample: Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.4 nm
Dmax 48.0 nm

SASDUY3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:32 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 12.2 nm

SASDUZ3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:16 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 14.5 nm

SASDU24 – Calcium activated full-length gelsolin and the F-form of actin at a 1:8 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.6 nm