Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDU86 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 7.0 mg/mL, 10 mM fructose

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

Glucokinase-1Glucokinase-1 experimental SAS data

Sample:	Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, 10 mM D-fructose, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier

2.7

SASDU96 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 7.0 mg/mL, 10 mM mannose

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

Sample:	Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, 10 mM D-mannose, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier	2.7	nm
D_max	10.0	nm

SASDUA6 – Kluyveromyces lactis glucokinase 1 mutant H304Q, 7.0 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (H304Q)

Glucokinase-1 (H304Q)Glucokinase-1 (H304Q) experimental SAS data

Sample:	Glucokinase-1 (H304Q) monomer, 54 kDa Kluyveromyces lactis (strain … protein Glucokinase-1 (H304Q) dimer, 107 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier

2.5

SASDUB6 – Kluyveromyces lactis glucokinase 1 mutant H304Q, 7.0 mg/mL, 10 mM fructose

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (H304Q)

Sample:	Glucokinase-1 (H304Q) monomer, 54 kDa Kluyveromyces lactis (strain … protein Glucokinase-1 (H304Q) dimer, 107 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, 10 mM D-fructose, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier

2.7

SASDUC6 – Kluyveromyces lactis glucokinase 1 mutant H304Q, 7.0 mg/mL, 10 mM mannose

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (H304Q)

Sample:	Glucokinase-1 (H304Q) monomer, 54 kDa Kluyveromyces lactis (strain … protein Glucokinase-1 (H304Q) dimer, 107 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, 10 mM D-mannose, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier

2.7

SASDUD6 – Extrapolated scattering curve of monomeric Kluyveromyces lactis glucokinase 1 triple point mutant I356D, Y419D, H420D

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (I356D, Y419D, H420D)

Glucokinase-1 (I356D, Y419D, H420D) experimental SAS data

Sample:	Glucokinase-1 (I356D, Y419D, H420D) monomer, 54 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier	2.6	nm
D_max	9.0	nm
Volume^Porod	78	nm³

SASDUE6 – Monomeric Kluyveromyces lactis glucokinase 1 triple point mutant (I356D, Y419D, H420D), 3.0 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (I356D, Y419D, H420D)

Sample:	Glucokinase-1 (I356D, Y419D, H420D) monomer, 54 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier	2.4	nm
D_max	8.0	nm
Volume^Porod	76	nm³

SASDUF6 – Monomeric Kluyveromyces lactis glucokinase 1 triple point mutant (I356D, Y419D, H420D), 5.0 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (I356D, Y419D, H420D)

Sample:	Glucokinase-1 (I356D, Y419D, H420D) monomer, 54 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier	2.5	nm
D_max	8.0	nm
Volume^Porod	75	nm³

SASDUG6 – Monomeric Kluyveromyces lactis glucokinase 1 triple point mutant (I356D, Y419D, H420D), 10.0 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (I356D, Y419D, H420D)

Sample:	Glucokinase-1 (I356D, Y419D, H420D) monomer, 54 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 13

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier

2.3

SASDUH6 – Monomeric Kluyveromyces lactis glucokinase 1 triple point mutant (I356D, Y419D, H420D), 2.5 mg/mL, 10 mM fructose

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1 (H304Q)

Glucokinase-1 (H304Q) experimental SAS data

Sample:	Glucokinase-1 (H304Q) monomer, 54 kDa Kluyveromyces lactis (strain … protein
Buffer:	10 mM Tris-HCl, 1 mM DTT, 10 mM D-fructose, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 14

Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße

R_g^Guinier	2.5	nm
D_max	8.2	nm
Volume^Porod	76	nm³

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