SASBDB entries for UniProt ID:

SASDVD2 – Human Haemoglobin (Sigma-Aldrich) with 5% (w/v) PEG4000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG4000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.1 nm
VolumePorod 91 nm3

SASDVE2 – Human Haemoglobin (Sigma-Aldrich) with 10% PEG2000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
DAMMIF model
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 10% (w/v) PEG2000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.8 nm
Dmax 11.3 nm
VolumePorod 131 nm3

SASDVV2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (76-549)

UniProt ID: Q14296 (76-549) Fas-activated serine/threonine kinase (amino acids 76-549)
Fas-activated serine/threonine kinase (amino acids 76-549) experimental SAS data
Sample: Fas-activated serine/threonine kinase (amino acids 76-549) monomer, 53 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31
Human FASTK protein
Daria Dawidziak
RgGuinier 3.6 nm
Dmax 16.3 nm
VolumePorod 96 nm3

SASDVW2 – Fas-activated serine/threonine kinase/phosphoprotein FASTK (169-549)

UniProt ID: Q14296 (169-549) Fas-activated serine/threonine kinase (amino acids 169-549)
Fas-activated serine/threonine kinase (amino acids 169-549) experimental SAS data
Sample: Fas-activated serine/threonine kinase (amino acids 169-549) monomer, 43 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 5% (v/v) Glycerol, 200 mM Ammonium Sulphate, 50 mM NaCl, 0.5 mM TCEP, 10 mM Glutamic acid, 10 mM Arginine;, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 31
Human FASTK protein
Daria Dawidziak
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 67 nm3

SASDVZ2 – Human Collagen Galactosyltransferase GLT25D1/COLGALT1

UniProt ID: Q8NBJ5 (30-618) Procollagen galactosyltransferase 1
Procollagen galactosyltransferase 1 experimental SAS data
CORAL model
Sample: Procollagen galactosyltransferase 1 dimer, 137 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 5
Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 Nature Communications 16(1) (2025)
De Marco M, Rai S, Scietti L, Mattoteia D, Liberi S, Moroni E, Pinnola A, Vetrano A, Iacobucci C, Santambrogio C, Colombo G, Forneris F
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 184 nm3

SASDVW3 – Lipoprotein NlpI dimer bound to two asymmetrical murein DD-endopeptidase mMepS dimers at pH 7.0

UniProt ID: P0AFB1 (20-294) Lipoprotein NlpI

UniProt ID: P0AFV4 (28-188) Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase
Lipoprotein NlpIMurein DD-endopeptidase MepS/Murein LD-carboxypeptidase experimental SAS data
PYMOL model
Sample: Lipoprotein NlpI dimer, 63 kDa Escherichia coli (strain … protein
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase tetramer, 73 kDa Escherichia coli (strain … protein
Buffer: 0.2 M Na/K phosphate pH 7.0, 2 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at TPS13A, NSRRC on 2023 Sep 7
Structural basis for recruitment of peptidoglycan endopeptidase MepS by lipoprotein NlpI. Nat Commun 15(1):5461 (2024)
Wang S, Huang CH, Lin TS, Yeh YQ, Fan YS, Wang SW, Tseng HC, Huang SJ, Chang YY, Jeng US, Chang CI, Tzeng SR
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 123 nm3

SASDVX3 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel DeCLIC with calcium at pH 5 by paused SEC-SANS

UniProt ID: V4JF97 (34-642) Neurotransmitter-gated ion-channel ligand-binding domain-containing protein
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
Desulfofustis sp. PB-SRB1 ligand-gated ion channel DeCLIC with calcium at pH 5 by paused SEC-SANS Rg histogram
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM citrate, 150 mM NaCl, 10 mM CaCl2, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2021 Jun 17
Structural characterization of pH-modulated closed and open states in a pentameric ligand-gated ion channel
Marie Lycksell
RgGuinier 5.1 nm
Dmax 17.1 nm
VolumePorod 581 nm3

SASDVY3 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel DeCLIC without calcium at pH 5 by paused SEC-SANS

UniProt ID: V4JF97 (34-642) Neurotransmitter-gated ion-channel ligand-binding domain-containing protein
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
Desulfofustis sp. PB-SRB1 ligand-gated ion channel DeCLIC without calcium at pH 5 by paused SEC-SANS Rg histogram
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM citrate, 150 mM NaCl, 10 mM EDTA, 0.5 mM deuterated n-Dodecyl-β-D-Maltoside, in D2O, pH: 5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2021 Jun 17
Structural characterization of pH-modulated closed and open states in a pentameric ligand-gated ion channel
Marie Lycksell
RgGuinier 5.2 nm
Dmax 18.4 nm
VolumePorod 558 nm3

SASDV54 – YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933 bound to a 22 base pair dsDNA.

UniProt ID: Q8XAD7 (1-100) Phage antirepressor protein Cro

UniProt ID: None (None-None) IR1 22 base pair dsDNA
Phage antirepressor protein CroIR1 22 base pair dsDNA experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Phage antirepressor protein Cro dimer, 24 kDa Escherichia coli O157:H7 protein
IR1 22 base pair dsDNA monomer, 14 kDa DNA
Buffer: 20 mM sodium acetate, 150 mM NaCl, 1 mM TCEP, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 13
Structural-function relationship of YdaS, a Cro-type repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7
Marusa Prolic Kalinsek
RgGuinier 2.8 nm
Dmax 10.1 nm
VolumePorod 53 nm3

SASDV64 – Serine hydrolase FphH with short chain esterase activity (Apo FphH)

UniProt ID: A0A0D6HZA6 (1-246) Alpha/beta fold hydrolase
Alpha/beta fold hydrolase experimental SAS data
DAMFILT model
Sample: Alpha/beta fold hydrolase monomer, 28 kDa Staphylococcus aureus protein
Buffer: 100 mM NaCl, 10 mM HEPES, pH: 7.6
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Apr 11
Similar but Distinct—Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH  and FphI Proteins: Structure, Function, and Bioinformatics (2024)
Fellner M, Randall G, Bitac I, Warrender A, Sethi A, Jelinek R, Kass I
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 41 nm3