SASBDB entries for UniProt ID:

SASDUW5 – C-terminal domain of Ubiquitinating/deubiquitinating enzyme SdeA (SdeA_CTD)

UniProt ID: Q5ZTK4 (1158-1499) Ubiquitinating/deubiquitinating enzyme SdeA
Ubiquitinating/deubiquitinating enzyme SdeA experimental SAS data
MONSA model
Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 39 kDa Legionella pneumophila subsp. … protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4
Structural Basis for Membrane Targeting and Secretion of Legionella SidE Ubiquitin Ligases
Ahmed Mohammed
RgGuinier 3.9 nm
Dmax 15.7 nm
VolumePorod 62 nm3

SASDUX5 – Type 4 adaptor protein complex IcmS, IcmW and DotL (IcmS/IcmW/DotLc)

UniProt ID: Q5ZYD0 (1-111) Type 4 adapter protein IcmS

UniProt ID: Q5ZS31 (1-151) Type 4 adapter protein IcmW (S9A)

UniProt ID: Q5ZYC6 (656-783) Type 4 coupling protein DotL
Type 4 adapter protein IcmSType 4 adapter protein IcmW (S9A)Type 4 coupling protein DotL experimental SAS data
MONSA model
Sample: Type 4 adapter protein IcmS monomer, 14 kDa Legionella pneumophila subsp. … protein
Type 4 adapter protein IcmW (S9A) monomer, 17 kDa Legionella pneumophila subsp. … protein
Type 4 coupling protein DotL monomer, 15 kDa Legionella pneumophila subsp. … protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4
Structural Basis for Membrane Targeting and Secretion of Legionella SidE Ubiquitin Ligases
Ahmed Mohammed
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 71 nm3

SASDUY5 – C-terminal domain of Ubiquitinating/deubiquitinating enzyme SdeA bound to Type 4 adaptor protein complex IcmS, IcmW and DotL (SdeACTD-IcmSWDotLc complex)

UniProt ID: Q5ZTK4 (1158-1499) Ubiquitinating/deubiquitinating enzyme SdeA

UniProt ID: Q5ZYD0 (1-111) Type 4 adapter protein IcmS

UniProt ID: Q5ZS31 (1-151) Type 4 adapter protein IcmW (S9A)

UniProt ID: Q5ZYC6 (656-783) Type 4 coupling protein DotL
Ubiquitinating/deubiquitinating enzyme SdeAType 4 adapter protein IcmSType 4 adapter protein IcmW (S9A)Type 4 coupling protein DotL experimental SAS data
SREFLEX model
Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 39 kDa Legionella pneumophila subsp. … protein
Type 4 adapter protein IcmS monomer, 14 kDa Legionella pneumophila subsp. … protein
Type 4 adapter protein IcmW (S9A) monomer, 17 kDa Legionella pneumophila subsp. … protein
Type 4 coupling protein DotL monomer, 15 kDa Legionella pneumophila subsp. … protein
Buffer: 25 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 4
Structural Basis for Membrane Targeting and Secretion of Legionella SidE Ubiquitin Ligases
Ahmed Mohammed
RgGuinier 3.8 nm
Dmax 14.4 nm
VolumePorod 166 nm3

SASDUZ5 – Extrapolated scattering curve of dimeric Kluyveromyces lactis glucokinase 1 (KlGlk1)

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1 experimental SAS data
DAMMIN model
Sample: Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.4 nm
Dmax 9.9 nm
VolumePorod 154 nm3

SASDU26 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 0.6 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.1 nm
Dmax 9.5 nm
VolumePorod 108 nm3

SASDU36 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 1.0 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.1 nm
Dmax 9.5 nm
VolumePorod 128 nm3

SASDU46 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 1.9 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 138 nm3

SASDU56 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 3.7 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.1 nm
Dmax 10.0 nm
VolumePorod 140 nm3

SASDU66 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 7.3 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier 3.0 nm
Dmax 10.0 nm

SASDU76 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 14.2 mg/mL, no ligand

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1

UniProt ID: Q6CUZ3 (1-481) Glucokinase-1
Glucokinase-1Glucokinase-1 experimental SAS data
PYMOL model
Sample: Glucokinase-1 monomer, 54 kDa Kluyveromyces lactis (strain … protein
Glucokinase-1 dimer, 108 kDa Kluyveromyces lactis (strain … protein
Buffer: 10 mM Tris-HCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 20
Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)
Renato H. Weiße
RgGuinier -1.0 nm