SASBDB entries for UniProt ID:

SASDV22 – BAM2 in 100 mM KCl (1 mg/mL)

UniProt ID: O65258 (56-542) Beta-amylase 2, chloroplastic
Beta-amylase 2, chloroplastic experimental SAS data
Beta-amylase 2, chloroplastic Kratky plot
Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 100 mM KCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17
Potassium cations expand the conformation ensemble of Arabidopsis thaliana β-amylase2 (BAM2). MicroPubl Biol 2024 (2024)
Sholes A, Asongakap R, Jaconski S, Monroe J, Berndsen CE
RgGuinier 4.7 nm
Dmax 15.1 nm
VolumePorod 218 nm3

SASDV32 – BAM2 in 100 mM KCl (2 mg/mL)

UniProt ID: O65258 (56-542) Beta-amylase 2, chloroplastic
Beta-amylase 2, chloroplastic experimental SAS data
Beta-amylase 2, chloroplastic Kratky plot
Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 100 mM KCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17
Potassium cations expand the conformation ensemble of Arabidopsis thaliana β-amylase2 (BAM2). MicroPubl Biol 2024 (2024)
Sholes A, Asongakap R, Jaconski S, Monroe J, Berndsen CE
RgGuinier 4.7 nm
Dmax 15.0 nm
VolumePorod 220 nm3

SASDV52 – Aspergillus niger beta-glucosidase (bgl1), native, glycosylated

UniProt ID: Q9P8F4 (1-860) beta-glucosidase
beta-glucosidase experimental SAS data
DAMMIN model
Sample: Beta-glucosidase dimer, 187 kDa Aspergillus niger protein
Buffer: 50 mM sodium acetate, pH: 5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jun 4
Aspergillus niger beta-glucosidase (bgl1)
Estella Yee
RgGuinier 4.4 nm
Dmax 13.9 nm
VolumePorod 277 nm3

SASDV62 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, SEC-SAXS

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100 mM Sodium Phosphate, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 May 24
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 105 nm3

SASDV72 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml, 1 day after SEC

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100 mM Sodium Phosphate, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 103 nm3

SASDV82 – Human Haemoglobin (Sigma-Aldrich) with 5% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 105 nm3

SASDV92 – Human Haemoglobin (Sigma-Aldrich) with 10% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 10% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 7.2 nm
VolumePorod 113 nm3

SASDVA2 – Human Haemoglobin (Sigma-Aldrich) with 20% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 20% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.6 nm
Dmax 7.6 nm
VolumePorod 113 nm3

SASDVB2 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml, 1 week after SEC

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG2000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 107 nm3

SASDVC2 – Human Haemoglobin (Sigma-Aldrich) with 5% PEG2000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit betaHemoglobin subunit alphaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit beta Hemoglobin subunit alpha Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG2000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.5 nm
VolumePorod 92 nm3