SASBDB entries for UniProt ID:

SASDMR7 – HOLO-serotransferrin at pH 7.0 in the presence of iron

UniProt ID: P02787 (1-698) Serotransferrin
Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
Campos-Escamilla C, Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 103 nm3

SASDNS7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H3/H4 histone oligomer complex

UniProt ID: F4J9Q6 (1-136) Peptidyl-prolyl cis-trans isomerase FKBP43

UniProt ID: P68431 (1-136) Histone H3.1

UniProt ID: P62805 (1-103) Histone H4
Peptidyl-prolyl cis-trans isomerase FKBP43Histone H3.1Histone H4 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Histone H3.1 dimer, 31 kDa Homo sapiens protein
Histone H4 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 4.4 nm
Dmax 14.1 nm
VolumePorod 226 nm3

SASDP42 – dark-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

UniProt ID: P74102 (2-317) Orange carotenoid-binding protein
Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 44 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

UniProt ID: A0A509AF36 (2-228) Methionine--tRNA ligase

UniProt ID: A0A509ARF0 (2-202) tRNA import protein tRIP

UniProt ID: A0A509AR09 (2-228) Glutamate--tRNA ligase
Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3

SASDRL5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant + heme

UniProt ID: P73129 (1-96) Ssr1698 protein
Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.2 nm
VolumePorod 29 nm3

SASDRF6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated EphB4 peptide

UniProt ID: P54760 (586-602) Ephrin type-B receptor 4

UniProt ID: P20936 (174-1047) Ras GTPase-activating protein 1
Ephrin type-B receptor 4Ras GTPase-activating protein 1 experimental SAS data
Ephrin type-B receptor 4 Ras GTPase-activating protein 1 Kratky plot
Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein
Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 176 nm3

SASDRV9 – Regulator of telomere elongation helicase 1, C - terminal

UniProt ID: Q9NZ71-6 (756-1300) Regulator of telomere elongation helicase 1 (Isoform 6, 756-1300)
Regulator of telomere elongation helicase 1 (Isoform 6, 756-1300) experimental SAS data
Regulator of telomere elongation helicase 1, C - terminal Rg histogram
Sample: Regulator of telomere elongation helicase 1 (Isoform 6, 756-1300) monomer, 59 kDa Homo sapiens protein
Buffer: 50 mM Hepes, 150 mM NaCl,10 (v/v)% glycerol, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 22
Structural and biochemical characterization of the C‐terminal region of the human RTEL 1 helicase Protein Science 33(9) (2024)
Cortone G, Graewert M, Kanade M, Longo A, Hegde R, González‐Magaña A, Chaves‐Arquero B, Blanco F, Napolitano L, Onesti S
RgGuinier 4.9 nm
Dmax 15.3 nm
VolumePorod 132 nm3

SASDSR2 – Zea mays PAIR1 coiled-coiled with N-terminal SUMO-tag

UniProt ID: A0A804LC01 (30-200) Protein PAIR1
Protein PAIR1 experimental SAS data
OTHER model
Sample: Protein PAIR1 tetramer, 132 kDa Zea mays protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 8.0 nm
Dmax 31.7 nm

SASDST8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 2 (pCaMKK2)

UniProt ID: Q96RR4 (93-517) Calcium/calmodulin-dependent protein kinase kinase 2
Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 2 Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 87 nm3

SASDT52 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM

UniProt ID: P0DTC9 (176-245) Nucleoprotein (L223P, L227P, L230P)
Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 12 nm3