SASBDB entries for UniProt ID:

SASDDW2 – EspG5-PE25/PPE41 complex from M. tuberculosis

UniProt ID: O53943 (None-None) EspG5 chaperone from Mycobacterium tuberculosis

UniProt ID: None (None-None) PE25 from Mycobacterium tuberculosis

UniProt ID: Q79FE1 (None-None) PPE41 from Mycobacterium tuberculosis
EspG5 chaperone from Mycobacterium tuberculosisPE25 from Mycobacterium tuberculosisPPE41 from Mycobacterium tuberculosis experimental SAS data
Sample: EspG5 chaperone from Mycobacterium tuberculosis monomer, 32 kDa Mycobacterium tuberculosis protein
PE25 from Mycobacterium tuberculosis monomer, 11 kDa Mycobacterium tuberculosis protein
PPE41 from Mycobacterium tuberculosis monomer, 22 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Feb 10
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 4.0 nm
Dmax 13.0 nm

SASDDX2 – EspG3-PE5/PPE4 complex from M. tuberculosis

UniProt ID: P9WJC6 (None-None) EspG3 chaperone from Mycobacterium tuberculosis

UniProt ID: L7N695 (None-None) PE5 from Mycobacterium tuberculosis

UniProt ID: P9WI43 (None-None) PPE4 from Mycobacterium tuberculosis
EspG3 chaperone from Mycobacterium tuberculosisPE5 from Mycobacterium tuberculosisPPE4 from Mycobacterium tuberculosis experimental SAS data
Sample: EspG3 chaperone from Mycobacterium tuberculosis, 34 kDa Mycobacterium tuberculosis protein
PE5 from Mycobacterium tuberculosis monomer, 10 kDa Mycobacterium tuberculosis protein
PPE4 from Mycobacterium tuberculosis monomer, 52 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Jun 15
Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems (2018)
Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
RgGuinier 4.0 nm
Dmax 14.2 nm

SASDDY2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig

UniProt ID: Q80XU8 (17-371) Leucine-rich repeat and fibronectin type-III domain-containing protein 4
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 4 dimer, 79 kDa Mus musculus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 13
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 183 nm3

SASDDZ2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn

UniProt ID: Q80XU8 (17-510) Leucine-rich repeat and fibronectin type-III domain-containing protein 4
Leucine-rich repeat and fibronectin type-III domain-containing protein 4 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 4 dimer, 109 kDa Mus musculus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 11
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 4.8 nm
Dmax 17.1 nm
VolumePorod 313 nm3

SASDD23 – Leucine-rich repeat and fibronectin type-III domain-containing protein 5: Fragment SALM5 LRR-Ig

UniProt ID: Q8BXA0 (18-376) Leucine-rich repeat and fibronectin type-III domain-containing protein 5
Leucine-rich repeat and fibronectin type-III domain-containing protein 5 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 5: Fragment SALM5 LRR-Ig Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 5 dimer, 82 kDa Mus musculus protein
Buffer: 30 mM Tris-Cl, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jun 8
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 155 nm3

SASDD33 – Toxin/Antitoxin complex from M. tuberculosis

UniProt ID: P9WLP9 (None-None) Mycobacterial cidal toxin

UniProt ID: P9WLP7 (None-None) Mycobacterial cidal antitoxin
Mycobacterial cidal toxinMycobacterial cidal antitoxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Mycobacterial cidal toxin hexamer, 121 kDa Mycobacterium tuberculosis protein
Mycobacterial cidal antitoxin hexamer, 76 kDa Mycobacterium tuberculosis protein
Buffer: 100 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death. Mol Cell (2019)
Freire DM, Gutierrez C, Garza-Garcia A, Grabowska AD, Sala AJ, Ariyachaokun K, Panikova T, Beckham KSH, Colom A, Pogenberg V, Cianci M, Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA, Neyrolles O
RgGuinier 4.1 nm
Dmax 11.4 nm
VolumePorod 262 nm3

SASDD43 – Mycobacterial suicide toxin (MbsT)

UniProt ID: P9WLP9 (1-186) Mycobacterial cidal toxin
Mycobacterial cidal toxin experimental SAS data
SREFLEX model
Sample: Mycobacterial cidal toxin monomer, 20 kDa Mycobacterium tuberculosis H37Rv protein
Buffer: 30 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 2
MbsTA
Diana Freire
RgGuinier 1.8 nm
Dmax 5.6 nm
VolumePorod 30 nm3

SASDD53 – Tetratrico peptide repeat domain of Bacterial cellulose synthesis subunit C

UniProt ID: K0J1W8 (24-664) Bacterial cellulose synthesis subunit C
Bacterial cellulose synthesis subunit C experimental SAS data
DAMMIF model
Sample: Bacterial cellulose synthesis subunit C monomer, 71 kDa Enterobacter sp. CJF-002 protein
Buffer: 50 mM HEPES, 100 mM KCl, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Apr 24
Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C. Sci Rep 7(1):13018 (2017)
Nojima S, Fujishima A, Kato K, Ohuchi K, Shimizu N, Yonezawa K, Tajima K, Yao M
RgGuinier 5.1 nm
Dmax 18.5 nm
VolumePorod 115 nm3

SASDD73 – Flavin reductase Pden_5119

UniProt ID: A1BCD5 (None-None) NADPH-dependent FMN reductase
NADPH-dependent FMN reductase experimental SAS data
SWISSMODEL model
Sample: NADPH-dependent FMN reductase dimer, 41 kDa Paracoccus denitrificans protein
Buffer: 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 Feb 22
Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden_5119) of Paracoccus denitrificans. Int J Mol Sci 24(4) (2023)
Kryl M, Sedláček V, Kučera I
RgGuinier 2.3 nm
Dmax 6.3 nm
VolumePorod 70 nm3

SASDD83 – Apolipoprotein D (ApoD) tetramer

UniProt ID: P05090 (21-189) Apolipoprotein D
Apolipoprotein D experimental SAS data
SASREF CV model
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Identification of a novel tetrameric structure for human apolipoprotein-D. J Struct Biol 203(3):205-218 (2018)
Kielkopf CS, Low JKK, Mok YF, Bhatia S, Palasovski T, Oakley AJ, Whitten AE, Garner B, Brown SHJ
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 169 nm3