SASBDB entries for UniProt ID:

SASDCR2 – Bromodomain-containing protein 4 (BRD4) tandem bromodomains

UniProt ID: O60885 (44-460) Bromodomain-containing protein 4
Bromodomain-containing protein 4 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 4 monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 251 nm3

SASDCK3 – Aldehyde dehydrogenase

UniProt ID: P0A9Q8 (1-445) Aldehyde-alcohol dehydrogenase
Aldehyde-alcohol dehydrogenase experimental SAS data
DAMMIF model
Sample: Aldehyde-alcohol dehydrogenase monomer, 48 kDa Escherichia coli O157:H7 protein
Buffer: 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 30
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020)
Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
RgGuinier 2.7 nm
Dmax 11.1 nm
VolumePorod 74 nm3

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

UniProt ID: A0A0D7C2L1 (2-92) Plasmid stabilization protein ParE

UniProt ID: A0A0F6F6Q9 (13-63) Uncharacterized protein
Plasmid stabilization protein ParEUncharacterized protein experimental SAS data
parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram
Sample: Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein
Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 2.2 nm
Dmax 9.3 nm
VolumePorod 36 nm3

SASDCT4 – Collagenase ColG s2s3as3b at pCa 4

UniProt ID: Q9X721 (787-1118) Collagenase ColG segement s2s3as3b
Collagenase ColG segement s2s3as3b experimental SAS data
DAMMIF model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 63 nm3

SASDDV9 – Mycobacterium tuberculosis DNA LigaseA with Nicked DNA

UniProt ID: P9WNV1 (None-None) DNA ligase A

UniProt ID: None (None-None) Nicked DNA
DNA ligase ANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Nicked DNA dimer, 16 kDa DNA
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. Nucleic Acids Res (2020)
Khanam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 4.4 nm
Dmax 14.8 nm
VolumePorod 262 nm3

SASDE22 – Protein translocase subunit SecA (amino acids 1-832)

UniProt ID: P10408 (1-832) Protein translocase subunit SecA
Protein translocase subunit SecA experimental SAS data
Protein translocase subunit SecA Kratky plot
Sample: Protein translocase subunit SecA dimer, 189 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, 1mM TCEP, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 18
The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity. Elife 8 (2019)
Jamshad M, Knowles TJ, White SA, Ward DG, Mohammed F, Rahman KF, Wynne M, Hughes GW, Kramer G, Bukau B, Huber D
RgGuinier 4.5 nm
Dmax 15.7 nm
VolumePorod 398 nm3

SASDER3 – Senescence-associated E3 ubiquitin ligase 1, 1.8 mg/ml

UniProt ID: Q9LM76 (None-None) Senescence-associated E3 ubiquitin ligase 1
Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 5.9 nm
Dmax 23.0 nm

SASDEL4 – Residues 478-884 of pseudorabies virus tegument protein UL37

UniProt ID: Q911W0 (478-884) Tegument protein UL37
Tegument protein UL37 experimental SAS data
DAMMIN model
Sample: Tegument protein UL37 monomer, 43 kDa Suid alphaherpesvirus 1 protein
Buffer: 100 mM HEPES 150 mM NaCl 5% glycerol 0.1 mM tris(2-carboxyethyl)phosphine (TCEP), pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Jun 3
The dynamic nature of the conserved tegument protein UL37 of herpesviruses. J Biol Chem 293(41):15827-15839 (2018)
Koenigsberg AL, Heldwein EE
RgGuinier 3.9 nm
Dmax 12.5 nm

SASDEQ4 – Deletion mutant of the suppressor of copper sensitivity C protein, PmScsC, with concentration series data

UniProt ID: A0A1Z1SYD5 (22-243) Deletion mutant of PmScsC
Deletion mutant of PmScsC experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Deletion mutant of PmScsC, 23 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis. Acta Crystallogr D Struct Biol 75(Pt 3):296-307 (2019)
Furlong EJ, Kurth F, Premkumar L, Whitten AE, Martin JL
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 54 nm3

SASDEX5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site I

UniProt ID: P01308 (25-110) Insulin detemir (Levemir(R), Novo Nordisk A/S)

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)
Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3