SASBDB entries for UniProt ID:

SASDE24 – Tryparedoxin, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

UniProt ID: O77404 (2-144) Tryparedoxin
Tryparedoxin experimental SAS data
SASREF MX model
Sample: Tryparedoxin, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 45 nm3

SASDE34 – Tryparedoxin W39A, reduced state

UniProt ID: O77404 (2-144) Tryparedoxin W39A
Tryparedoxin W39A experimental SAS data
OTHER model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3

SASDE44 – Tryparedoxin W39A, oxidized state

UniProt ID: O77404 (2-144) Tryparedoxin W39A
Tryparedoxin W39A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3

SASDE54 – Tryparedoxin W39A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

UniProt ID: O77404 (2-144) Tryparedoxin W39A
Tryparedoxin W39A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W39A, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.8 nm
VolumePorod 26 nm3

SASDE64 – Tryparedoxin W70A, reduced state

UniProt ID: O77404 (None-None) Tryparedoxin W70A
Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 4.9 nm
VolumePorod 26 nm3

SASDE74 – Tryparedoxin W70A, oxidized state

UniProt ID: O77404 (None-None) Tryparedoxin W70A
Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.7 nm
Dmax 7.7 nm
VolumePorod 31 nm3

SASDE84 – Tryparedoxin W70A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

UniProt ID: O77404 (None-None) Tryparedoxin W70A
Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 7.2 nm
VolumePorod 42 nm3

SASDE94 – Tryparedoxin I109A, reduced state

UniProt ID: O77404 (None-None) Tryparedoxin I109A
Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 25 nm3

SASDEA4 – Tryparedoxin I109A, oxidized state

UniProt ID: O77404 (None-None) Tryparedoxin I109A
Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.7 nm
Dmax 4.3 nm
VolumePorod 27 nm3

SASDEB4 – Tryparedoxin I109A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

UniProt ID: O77404 (None-None) Tryparedoxin I109A
Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 42 nm3