SASBDB entries for UniProt ID:

SASDEA3 – Labeled thioredoxin (TRX-Alexa488/Alexa594) with denaturant

UniProt ID: P0AA25 (2-106) Thioredoxin 1

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Thioredoxin 1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Thioredoxin 1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 13.9 nm
VolumePorod 31 nm3

SASDEB3 – Unlabeled nuclear pore complex protein Nup98-Nup96 (N98) without denaturant

UniProt ID: P52948 (2-150) Nuclear pore complex protein Nup98-Nup96
Nuclear pore complex protein Nup98-Nup96 experimental SAS data
Nuclear pore complex protein Nup98-Nup96 Kratky plot
Sample: Nuclear pore complex protein Nup98-Nup96 monomer, 15 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 10.4 nm
VolumePorod 27 nm3

SASDEC3 – Unlabeled nucleoporin NSP1 (NSP) without denaturant

UniProt ID: P14907 (2-175) Nucleoporin NSP1
Nucleoporin NSP1 experimental SAS data
Nucleoporin NSP1 Kratky plot
Sample: Nucleoporin NSP1 monomer, 18 kDa Saccharomyces cerevisiae protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 45 nm3

SASDEH3 – TubR protein of the pXO1-like plasmid pBc10987 from B. cereus (Bc-TubR) bound to S48 DNA (Bc-TubR : S48 DNA complex)

UniProt ID: None (None-None) S48 DNA strand 1

UniProt ID: None (None-None) S48 DNA strand 2

UniProt ID: B7JTH0 (1-116) TubR of the pXO1-like plasmid pBc10987 from B. cereus (Bc-TubR)
S48 DNA strand 1S48 DNA strand 2TubR of the pXO1-like plasmid pBc10987 from B. cereus (Bc-TubR) experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: S48 DNA strand 1 monomer, 21 kDa DNA
S48 DNA strand 2 monomer, 21 kDa DNA
TubR of the pXO1-like plasmid pBc10987 from B. cereus (Bc-TubR) decamer, 137 kDa protein
Buffer: 0.1 M NaCl, 10 mM Tris, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Nov 28
Cooperative DNA Binding of the Plasmid Partitioning Protein TubR from the Bacillus cereus pXO1 Plasmid. J Mol Biol (2018)
Hayashi I, Oda T, Sato M, Fuchigami S
RgGuinier 6.1 nm
Dmax 23.0 nm
VolumePorod 305 nm3

SASDEJ3 – Truncated neutophil cytosol factor 1, p47phox [1-342]

UniProt ID: P14598 (1-342) Neutophil cytosol factor 1
Neutophil cytosol factor 1 experimental SAS data
OTHER model
Sample: Neutophil cytosol factor 1 monomer, 40 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2009 Oct 16
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 58 nm3

SASDEK3 – The neutrophil cytosol factor 1 (p47phox) subunit of phagocyte NADPH oxidase

UniProt ID: P14598 (1-390) Neutrophil cytosol factor 1
Neutrophil cytosol factor 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Neutrophil cytosol factor 1 monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2008 Apr 23
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 77 nm3

SASDEL3 – The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase

UniProt ID: P19878 (1-526) Neutrophil cytosol factor 2
Neutrophil cytosol factor 2 experimental SAS data
The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase Rg histogram
Sample: Neutrophil cytosol factor 2 monomer, 61 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 8
Experiment: SAXS data collected at D24, LURE on 2003 Apr 9
Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 113 nm3

SASDEM3 – Dimeric class D beta-lactamase OXA-48

UniProt ID: Q6XEC0 (22-265) Beta-lactamase
Beta-lactamase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-lactamase dimer, 56 kDa Klebsiella pneumoniae protein
Buffer: 50 mM HEPES 50 mM K2SO4, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 23
The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity. FEBS J (2018)
Lund BA, Thomassen AM, Nesheim BHB, Carlsen TJO, Isaksson J, Christopeit T, Leiros HS
RgGuinier 2.5 nm
Dmax 7.4 nm
VolumePorod 74 nm3

SASDEY3 – Tryparedoxin, reduced state

UniProt ID: O77404 (None-None) Tryparedoxin
Tryparedoxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.8 nm
VolumePorod 27 nm3

SASDEZ3 – Tryparedoxin, oxidized state

UniProt ID: O77404 (None-None) Tryparedoxin
Tryparedoxin experimental SAS data
GASBOR model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.5 nm
VolumePorod 27 nm3