SASBDB entries for UniProt ID: P02768

SASDAA6 – Human serum albumin monomer and mixtures

UniProt ID: P02768 (25-609) Human serum albumin monomer
Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: Human serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

UniProt ID: P01241 (27-217) somapacitan

UniProt ID: P02768 (25-609) FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A

UniProt ID: P61768 (21-119) neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag

UniProt ID: P55899 (24-297) neonatal Fc receptor ectodomain alpha-chain
somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: Somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

UniProt ID: P02768 (25-609) FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A

UniProt ID: P61768 (21-119) neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag

UniProt ID: None (None-None) Albumin-binding side-chain

UniProt ID: P55899 (24-297) neonatal Fc receptor ectodomain alpha-chain
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDF42 – Human serum albumin in complex with somapacitan

UniProt ID: P02768 (25-609) Human serum albumin

UniProt ID: P01241 (27-217) Somapacitan
Human serum albuminSomapacitan experimental SAS data
SASREF model
Sample: Human serum albumin monomer, 66 kDa Homo sapiens protein
Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer: 100 mM MES, 140 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 202 nm3

SASDHV8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 267 nm3

SASDHW8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 274 nm3

SASDHX8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 258 nm3

SASDHY8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 5.0 nm
Dmax 17.4 nm
VolumePorod 270 nm3

SASDHZ8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM TRIS, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 239 nm3

SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

UniProt ID: P08473 (52-750) Neprilysin - G400V mutant

UniProt ID: P02768 (25-609) Human serum albumin - C58S mutant
Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM phosphate, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 240 nm3