SASBDB entries for UniProt ID: P0DTC9

SASDTJ5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 299 µM

UniProt ID: P0DTC9 (1-365) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein hexamer, 245 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 7.1 nm
Dmax 29.0 nm
VolumePorod 708 nm3

SASDPQ6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 38 nm3

SASDTX5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 19 µM

UniProt ID: P0DTC9 (1-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.3 nm
Dmax 26.0 nm
VolumePorod 233 nm3

SASDPR6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 51 nm3

SASDTY5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 50 µM

UniProt ID: P0DTC9 (1-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.9 nm
Dmax 28.5 nm
VolumePorod 571 nm3

SASDPS6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) AU extension in the 5'-genomic end of SARS-CoV-2
NucleoproteinAU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 32 nm3

SASDTZ5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 103 µM

UniProt ID: P0DTC9 (1-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein hexamer, 281 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 7.6 nm
Dmax 32.0 nm
VolumePorod 764 nm3

SASDPT6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions (mixture)

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 4 in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 25 nm3

SASDT26 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 216 µM

UniProt ID: P0DTC9 (1-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein octamer, 375 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 8.2 nm
Dmax 33.0 nm
VolumePorod 1028 nm3

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein
Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 26 nm3