SASBDB entries for UniProt ID: P0DTC9

SASDX56 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.4 nm
Dmax 166.0 nm

SASDX66 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 19.3 nm

SASDX76 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 2 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.5 nm
Dmax 17.8 nm

SASDX86 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 3 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.9 nm
Dmax 19.8 nm

SASDX96 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 4 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.9 nm
Dmax 23.5 nm

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein
Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 1.6 nm
Dmax 6.1 nm
VolumePorod 23 nm3

SASDTE5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 26 µM

UniProt ID: P0DTC9 (1-365) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 82 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.7 nm
Dmax 23.0 nm
VolumePorod 160 nm3

SASDTF5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 49 µM

UniProt ID: P0DTC9 (1-365) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.8 nm
Dmax 24.0 nm
VolumePorod 181 nm3

SASDTG5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 103 µM

UniProt ID: P0DTC9 (1-365) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.2 nm
Dmax 25.0 nm
VolumePorod 215 nm3

SASDTH5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 206 µM

UniProt ID: P0DTC9 (1-365) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 6.8 nm
Dmax 28.0 nm
VolumePorod 578 nm3