SASBDB entries for UniProt ID: P21333

SASDAE8 – Human Filamin A Ig-like domains 20-21 truncation (2151-2329) in complex with migfilin peptide

UniProt ID: P21333 (2151-2329) Human Filamin A Ig-like domains 20-21/migfilin peptide complex
Human Filamin A Ig-like domains 20-21/migfilin peptide complex experimental SAS data
Human Filamin A Ig-like domains 20-21/migfilin peptide complex Kratky plot
Sample: Human Filamin A Ig-like domains 20-21/migfilin peptide complex monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 1
Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21. PLoS One 10(8):e0136969 (2015)
Seppälä J, Tossavainen H, Rodic N, Permi P, Pentikäinen U, Ylänne J
RgGuinier 2.4 nm
Dmax 8.2 nm
VolumePorod 31 nm3

SASDAF8 – Human Filamin A Ig-like domains 20-21 truncation (2151-2329)

UniProt ID: P21333 (2151-2329) Human Filamin A Ig-like domains 20-21
Human Filamin A Ig-like domains 20-21 experimental SAS data
Human Filamin A Ig-like domains 20-21 Kratky plot
Sample: Human Filamin A Ig-like domains 20-21 monomer, 19 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 1
Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21. PLoS One 10(8):e0136969 (2015)
Seppälä J, Tossavainen H, Rodic N, Permi P, Pentikäinen U, Ylänne J
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 29 nm3

SASDAG8 – Human Filamin A Ig-like domains 20-21* truncation (2141-2329) in complex with migfilin peptide

UniProt ID: P21333 (2141-2329) Human Filamin A Ig-like domains 20-21*/migfilin peptide complex
Human Filamin A Ig-like domains 20-21*/migfilin peptide complex experimental SAS data
Human Filamin A Ig-like domains 20-21*/migfilin peptide complex Kratky plot
Sample: Human Filamin A Ig-like domains 20-21*/migfilin peptide complex monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 1
Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21. PLoS One 10(8):e0136969 (2015)
Seppälä J, Tossavainen H, Rodic N, Permi P, Pentikäinen U, Ylänne J
RgGuinier 2.4 nm
Dmax 8.2 nm
VolumePorod 33 nm3

SASDAH8 – Human Filamin A Ig-like domains 20-21* truncation (2141-2329)

UniProt ID: P21333 (2141-2329) Human Filamin A Ig-like domains 20-21*
Human Filamin A Ig-like domains 20-21* experimental SAS data
DAMMIF model
Sample: Human Filamin A Ig-like domains 20-21* monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Feb 1
Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21. PLoS One 10(8):e0136969 (2015)
Seppälä J, Tossavainen H, Rodic N, Permi P, Pentikäinen U, Ylänne J
RgGuinier 1.9 nm
Dmax 6.8 nm
VolumePorod 32 nm3

SASDB32 – Human Filamin A Ig-like domains 16-17 truncation (1782-1956)

UniProt ID: P21333 (1782-1956) Human Filamin A Ig-like domains 16-17
Human Filamin A Ig-like domains 16-17 experimental SAS data
DAMMIN model
Sample: Human Filamin A Ig-like domains 16-17 monomer, 19 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10 mM DTT, pH:
Experiment: SAXS data collected at BM29, ESRF on 2013 Sep 30
Skeletal Dysplasia Mutations Effect on Human Filamins' Structure and Mechanosensing. Sci Rep 7(1):4218 (2017)
Seppälä J, Bernardi RC, Haataja TJK, Hellman M, Pentikäinen OT, Schulten K, Permi P, Ylänne J, Pentikäinen U
RgGuinier 1.8 nm
Dmax 6.2 nm
VolumePorod 31 nm3

SASDB42 – Human Filamin A Ig-like domains 16-17* truncation (1782-1956) L1788R patient mutation

UniProt ID: P21333 (1781-1956) Human Filamin A Ig-like domains 16-17*
Human Filamin A Ig-like domains 16-17* experimental SAS data
DAMMIN model
Sample: Human Filamin A Ig-like domains 16-17* monomer, 19 kDa Homo sapiens protein
Buffer: 20 mM Tris 50 mM NaCl 10 mM DTT, pH:
Experiment: SAXS data collected at BM29, ESRF on 2013 Sep 30
Skeletal Dysplasia Mutations Effect on Human Filamins' Structure and Mechanosensing. Sci Rep 7(1):4218 (2017)
Seppälä J, Bernardi RC, Haataja TJK, Hellman M, Pentikäinen OT, Schulten K, Permi P, Ylänne J, Pentikäinen U
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 37 nm3

SASDFD3 – Filamin A Ig-like domains 4-6 (FLNa4-6)

UniProt ID: P21333 (574-869) Filamin A Ig-like domains 4-6
Filamin A Ig-like domains 4-6 experimental SAS data
DAMFILT model
Sample: Filamin A Ig-like domains 4-6 monomer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia. Biophys J 117(8):1467-1475 (2019)
Haataja TJK, Capoulade R, Lecointe S, Hellman M, Merot J, Permi P, Pentikäinen U
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 41 nm3

SASDFE3 – Filamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 4 mg/ml

UniProt ID: P21333 (574-869) Filamin A Ig-like domains 4-6, V711D mutation
Filamin A Ig-like domains 4-6, V711D mutation experimental SAS data
Filamin A Ig-like domains 4-6, V711D mutation Kratky plot
Sample: Filamin A Ig-like domains 4-6, V711D mutation monomer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia. Biophys J 117(8):1467-1475 (2019)
Haataja TJK, Capoulade R, Lecointe S, Hellman M, Merot J, Permi P, Pentikäinen U
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 64 nm3

SASDFF3 – Filamin A Ig-like domains 4-6, V711D mutant (FLNa4-6 V711D) at 2 mg/ml

UniProt ID: P21333 (574-869) Filamin A Ig-like domains 4-6, V711D mutation
Filamin A Ig-like domains 4-6, V711D mutation experimental SAS data
Filamin A Ig-like domains 4-6, V711D mutation Kratky plot
Sample: Filamin A Ig-like domains 4-6, V711D mutation monomer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia. Biophys J 117(8):1467-1475 (2019)
Haataja TJK, Capoulade R, Lecointe S, Hellman M, Merot J, Permi P, Pentikäinen U
RgGuinier 4.0 nm
Dmax 14.8 nm
VolumePorod 57 nm3

SASDFG3 – Filamin A Ig-like domains 4-6, H743P mutant (FLNa4-6 H743P) at 4 mg/ml

UniProt ID: P21333 (574-869) Filamin A Ig-like domains 4-6, H743P mutation
Filamin A Ig-like domains 4-6, H743P mutation experimental SAS data
Filamin A Ig-like domains 4-6, H743P mutation Kratky plot
Sample: Filamin A Ig-like domains 4-6, H743P mutation monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Critical Structural Defects Explain Filamin A Mutations Causing Mitral Valve Dysplasia. Biophys J 117(8):1467-1475 (2019)
Haataja TJK, Capoulade R, Lecointe S, Hellman M, Merot J, Permi P, Pentikäinen U
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 70 nm3