Browse by DISSEMINATION: Published

SASDQP4 – CRISPR associated Lon protease (CalpL) in the presence of cyclic oligoadenylate cA4

SAVED domain-containing protein experimental SAS data
DAMMIF model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 82 nm3

SASDQQ4 – CRISPR associated Lon protease (CalpL) asymmetrically dimerizing in the presence of cyclic oligoadenylate cA4

SAVED domain-containing protein experimental SAS data
SASREF MX model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 116 nm3

SASDM47 – NAD glycohydrolase (NADase)

NAD glycohydrolase experimental SAS data
BILBOMD model
Sample: NAD glycohydrolase monomer, 47 kDa Streptococcus pyogenes M1 … protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 16
Structural basis underlying the synergism of NADase and SLO during group A Streptococcus infection. Commun Biol 6(1):124 (2023)
Tsai WJ, Lai YH, Shi YA, Hammel M, Duff AP, Whitten AE, Wilde KL, Wu CM, Knott R, Jeng US, Kang CY, Hsu CY, Wu JL, Tsai PJ, Chiang-Ni C, Wu JJ, Lin YS, Liu CC, Senda T, Wang S
RgGuinier 3.0 nm
Dmax 103.0 nm
VolumePorod 66 nm3

SASDM57 – NAD glycohydrolase (NADase)/Streptolysin O (SLO) complex (SAXS with additional contrast variation SANS data)

NAD glycohydrolaseStreptolysin O (T66M) experimental SAS data
BILBOMD model
Sample: NAD glycohydrolase monomer, 47 kDa Streptococcus pyogenes M1 … protein
Streptolysin O (T66M) monomer, 63 kDa Streptococcus pyogenes serotype … protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 16
Structural basis underlying the synergism of NADase and SLO during group A Streptococcus infection. Commun Biol 6(1):124 (2023)
Tsai WJ, Lai YH, Shi YA, Hammel M, Duff AP, Whitten AE, Wilde KL, Wu CM, Knott R, Jeng US, Kang CY, Hsu CY, Wu JL, Tsai PJ, Chiang-Ni C, Wu JJ, Lin YS, Liu CC, Senda T, Wang S
RgGuinier 4.8 nm
Dmax 18.4 nm
VolumePorod 125 nm3

SASDPU6 – Duplex-G4-duplex DNA

Duplex-G4-duplex experimental SAS data
AMBER model
Sample: Duplex-G4-duplex monomer, 29 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 16
Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM. Nucleic Acids Res (2023)
Monsen RC, Chua EYD, Hopkins JB, Chaires JB, Trent JO
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 59 nm3

SASDPV3 – Wild type oxalyl-CoA synthetase Pcs60p (2.5 mg/ml) - hexamer-tetramer equilibrium

Oxalate--CoA ligase experimental SAS data
PYMOL model
Sample: Oxalate--CoA ligase, 363 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18
Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase. Biol Chem (2023)
Bürgi J, Lill P, Giannopoulou EA, Jeffries CM, Chojnowski G, Raunser S, Gatsogiannis C, Wilmanns M
RgGuinier 4.7 nm
Dmax 13.2 nm
VolumePorod 565 nm3

SASDPW3 – Wild type oxalyl-CoA synthetase Pcs60p (0.5 mg/ml) - tetramer-hexamer equilibrium

Oxalate--CoA ligase experimental SAS data
PYMOL model
Sample: Oxalate--CoA ligase, 363 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18
Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase. Biol Chem (2023)
Bürgi J, Lill P, Giannopoulou EA, Jeffries CM, Chojnowski G, Raunser S, Gatsogiannis C, Wilmanns M
RgGuinier 4.5 nm
Dmax 13.7 nm
VolumePorod 432 nm3

SASDPX3 – Point mutant K352D oxalyl-CoA synthetase Pcs60p (2.5 mg/ml) - dimer

Oxalate--CoA ligase (K352D) experimental SAS data
PYMOL model
Sample: Oxalate--CoA ligase (K352D) dimer, 121 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18
Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase. Biol Chem (2023)
Bürgi J, Lill P, Giannopoulou EA, Jeffries CM, Chojnowski G, Raunser S, Gatsogiannis C, Wilmanns M
RgGuinier 3.3 nm
Dmax 11.1 nm
VolumePorod 163 nm3

SASDPY3 – Point mutant K352D oxalyl-CoA synthetase Pcs60p (0.5 mg/ml) - dimer

Oxalate--CoA ligase (K352D) experimental SAS data
PYMOL model
Sample: Oxalate--CoA ligase (K352D) dimer, 121 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 50 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 18
Asymmetric horseshoe-like assembly of peroxisomal yeast oxalyl-CoA synthetase. Biol Chem (2023)
Bürgi J, Lill P, Giannopoulou EA, Jeffries CM, Chojnowski G, Raunser S, Gatsogiannis C, Wilmanns M
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 194 nm3

SASDHH7 – Upstream of N-ras, isoform A, CSD 7, 8 and 9 from Drosophila melanogaster

Upstream of N-ras, isoform A experimental SAS data
Upstream of N-ras, isoform A Kratky plot
Sample: Upstream of N-ras, isoform A monomer, 26 kDa Drosophila melanogaster protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 19
Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein. Nucleic Acids Res (2023)
Hollmann NM, Jagtap PKA, Linse JB, Ullmann P, Payr M, Murciano B, Simon B, Hub JS, Hennig J
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 38 nm3