Small Angle Scattering Biological Data Bank SASBDB

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SASDLV6 – High mannose glycan contactin 1 ectodomain, 20.5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B

R_g^Guinier	7.0	nm
D_max	34.0	nm
Volume^Porod	285	nm³

SASDLW6 – High mannose glycan contactin 1 ectodomain, 11.1 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	27.0	nm
Volume^Porod	275	nm³

SASDLX6 – High mannose glycan contactin 1 ectodomain, 5.5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	27.0	nm
Volume^Porod	270	nm³

SASDLY6 – High mannose glycan contactin 1 ectodomain, 2.4 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	19.5	nm
Volume^Porod	254	nm³

SASDFE6 – Sliding beta clamp

Beta sliding clamp experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

Regulation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex International Journal of Biological Macromolecules (2022)
Shukla A, Afsar M, Khanam T, Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R

R_g^Guinier	3.7	nm
D_max	10.3	nm
Volume^Porod	186	nm³

SASDFF6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp complex

Beta sliding clampProbable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

R_g^Guinier	3.5	nm
D_max	11.3	nm
Volume^Porod	98	nm³

SASDFG6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex

Beta sliding clampProbable exodeoxyribonuclease III protein XthADNA ligase A experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	25.3	nm
Volume^Porod	501	nm³

SASDFH6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate

Probable exodeoxyribonuclease III protein XthADNA ligase ABeta sliding clampDNA ligase A nicked DNA substrate experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein DNA ligase A nicked DNA substrate dimer, 16 kDa DNA
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	19.1	nm
Volume^Porod	496	nm³

SASDPF3 – Receptor-type tyrosine-protein phosphatase kappa extracellular domains (PTPRK-ECDs)

Receptor-type tyrosine-protein phosphatase kappa experimental SAS data

Sample:	Receptor-type tyrosine-protein phosphatase kappa monomer, 82 kDa Homo sapiens protein
Buffer:	50 mM MES, 250 mM NaCl, 3% v/v glycerol,, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Dec 13

Determinants of receptor tyrosine phosphatase homophilic adhesion: structural comparison of PTPRK and PTPRM extracellular domains Journal of Biological Chemistry :102750 (2022)
Hay I, Shamin M, Caroe E, Mohammed A, Svergun D, Jeffries C, Graham S, Sharpe H, Deane J

R_g^Guinier	7.0	nm
D_max	26.0	nm
Volume^Porod	252	nm³

SASDPG3 – Receptor-type tyrosine-protein phosphatase mu extracellular domains (PTPRM-ECDs)

Receptor-type tyrosine-protein phosphatase mu experimental SAS data

Sample:	Receptor-type tyrosine-protein phosphatase mu monomer, 82 kDa Homo sapiens protein
Buffer:	50 mM MES, 250 mM NaCl, 3% v/v glycerol,, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Dec 13

R_g^Guinier	7.2	nm
D_max	26.0	nm
Volume^Porod	255	nm³

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