Browse by DISSEMINATION: Published

SASDP46 – Glycoside Hydrolase Family 5 endo-mannanase retrieved from Capybara gut metagenome

GH5 endo-mannanase from Capybara gut metagenome experimental SAS data
PYMOL model
Sample: GH5 endo-mannanase from Capybara gut metagenome monomer, 46 kDa metagenome protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 Oct 30
Glycoside hydrolase subfamily GH5_57 features a highly redesigned catalytic interface to process complex hetero-β-mannans. Acta Crystallogr D Struct Biol 78(Pt 11):1358-1372 (2022)
Martins MP, Morais MAB, Persinoti GF, Galinari RH, Yu L, Yoshimi Y, Passos Nunes FB, Lima TB, Barbieri SF, Silveira JLM, Lombard V, Terrapon N, Dupree P, Henrissat B, Murakami MT
RgGuinier 2.3 nm
Dmax 5.5 nm
VolumePorod 58 nm3

SASDQG6 – N-terminal truncation mutant of the periplasmic region of conjugal transfer mating pair stabilization protein TraG* from the R100 plasmid

Conjugal transfer mating pair stabilization protein TraG experimental SAS data
DAMMIF model
Sample: Conjugal transfer mating pair stabilization protein TraG monomer, 52 kDa Shigella flexneri 4c protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23
Solution characterization of the dynamic conjugative entry exclusion protein TraG Structural Dynamics 9(6):064702 (2022)
Bragagnolo N, Audette G
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 130 nm3

SASDQH6 – Periplasmic region of the conjugal transfer mating pair stabilization protein TraG* from the R100 plasmid

Conjugal transfer mating pair stabilization protein TraG experimental SAS data
DAMMIF model
Sample: Conjugal transfer mating pair stabilization protein TraG monomer, 57 kDa Shigella flexneri 4c protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 0.05% NP40, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 23
Solution characterization of the dynamic conjugative entry exclusion protein TraG Structural Dynamics 9(6):064702 (2022)
Bragagnolo N, Audette G
RgGuinier 5.8 nm
Dmax 45.0 nm

SASDNA9 – Acinetobacter baumannii putrescine N-hydroxylase, 1 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 394 nm3

SASDNB9 – Acinetobacter baumannii putrescine N-hydroxylase, 3 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 390 nm3

SASDNC9 – Acinetobacter baumannii putrescine N-hydroxylase, 5 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 390 nm3

SASDND9 – Acinetobacter baumannii putrescine N-hydroxylase, 6 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.2 nm
Dmax 15.4 nm
VolumePorod 390 nm3

SASDNE9 – Acinetobacter baumannii putrescine N-hydroxylase, 8 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.2 nm
Dmax 15.5 nm
VolumePorod 390 nm3

SASDNF9 – Acinetobacter baumannii putrescine N-hydroxylase, 9 mg/mL

L-lysine 6-monooxygenase (NADPH-requiring) experimental SAS data
ALLOSMOD model
Sample: L-lysine 6-monooxygenase (NADPH-requiring) tetramer, 214 kDa Acinetobacter baumannii MRSN … protein
Buffer: 25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 14
Kinetic and Structural Characterization of a Flavin-Dependent Putrescine N-Hydroxylase from Acinetobacter baumannii. Biochemistry (2022)
Lyons NS, Bogner AN, Tanner JJ, Sobrado P
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 396 nm3

SASDQN5 – Candida glabrata Metacaspase in 10 mM CaCl2

Metacaspase-1 experimental SAS data
Metacaspase-1 Kratky plot
Sample: Metacaspase-1 monomer, 46 kDa Candida glabrata (strain … protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1% glycerol, 10 mM CaCl2, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Sep 26
Structural and molecular determinants of Candida glabrata metacaspase maturation and activation by calcium. Commun Biol 5(1):1158 (2022)
Conchou L, Doumèche B, Galisson F, Violot S, Dugelay C, Diesis E, Page A, Bienvenu AL, Picot S, Aghajari N, Ballut L
RgGuinier 1.9 nm
Dmax 5.4 nm
VolumePorod 43 nm3