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SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence

Transposon Tn3 resolvaseTn3 res, resolvase binding site II experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein
Tn3 res, resolvase binding site II monomer, 32 kDa DNA
Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6
Structural basis for topological regulation of Tn3 resolvase. Nucleic Acids Res (2022)
Montaño SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
RgGuinier 5.0 nm
Dmax 14.9 nm
VolumePorod 217 nm3

SASDPD4 – Ca2+-calmodulin

Calmodulin-1 experimental SAS data
Calmodulin-1 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 2.1 nm
Dmax 6.9 nm
VolumePorod 23 nm3

SASDPE4 – P2X7 ballast domain

P2X purinoceptor 7 experimental SAS data
P2X purinoceptor 7 Kratky plot
Sample: P2X purinoceptor 7 trimer, 74 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 94 nm3

SASDPF4 – P2X7 ballast domain in complex with Ca2+-calmodulin

Calmodulin-1P2X purinoceptor 7 experimental SAS data
Calmodulin-1 P2X purinoceptor 7 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 73 nm3

SASDP39 – apo Saccharomyces cerevisiae Arginyltransferase-1

Arginyl-tRNA--protein transferase 1 experimental SAS data
ALPHAFOLD model
Sample: Arginyl-tRNA--protein transferase 1 monomer, 60 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 2% glycerol, 100 mM KCl, 50 mM Tris-HCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 12
The Structure of Saccharomyces cerevisiae Arginyltransferase 1 (ATE1). J Mol Biol 434(21):167816 (2022)
Van V, Ejimogu NE, Bui TS, Smith AT
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 105 nm3

SASDNN5 – Meiosis protein TEX12 (Testis-expressed protein 12) – wild-type

Testis-expressed protein 12 experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 dimer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 27 nm3

SASDNP5 – Meiosis protein TEX12 (Testis-expressed protein 12) – delta Ctip

Testis-expressed protein 12 experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 tetramer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 44 nm3

SASDNQ5 – Meiosis protein TEX12 (Testis-expressed protein 12) – L110E, F114E, I117E, L121E mutant

Testis-expressed protein 12 (L110E, F114E, I117E, L121E) experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 (L110E, F114E, I117E, L121E) tetramer, 36 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 15
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 3.3 nm
Dmax 12.9 nm
VolumePorod 59 nm3

SASDNR5 – Meiosis protein TEX12 (Testis-expressed protein 12) – F102A, F109E, V116A mutant

Testis-expressed protein 12 (F102A, F109E, V116A) experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 (F102A, F109E, V116A) tetramer, 36 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 27
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 48 nm3

SASDNS5 – Meiosis protein TEX12 (Testis-expressed protein 12) – F109E mutant

Testis-expressed protein 12 (F109E) experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 (F109E) dimer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 13
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 30 nm3