Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDR78 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinic acid

Nicotinamide phosphoribosyltransferase Δ42-51 experimental SAS data

Nicotinamide phosphoribosyltransferase Δ42-51 Kratky plot

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M

R_g^Guinier	3.3	nm
D_max	11.2	nm
Volume^Porod	152	nm³

SASDR88 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of phosphoribosyl pyrophosphate

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

R_g^Guinier	3.2	nm
D_max	11.4	nm
Volume^Porod	154	nm³

SASDS36 – Human RAD51C-XRCC3 at pH 8 in the presence of ATP and vanadate, a phosphate mimic

Isoform 1 of DNA repair protein RAD51 homolog 3DNA repair protein XRCC3 experimental SAS data

Sample:	Isoform 1 of DNA repair protein RAD51 homolog 3 monomer, 40 kDa Homo sapiens protein DNA repair protein XRCC3 monomer, 38 kDa Homo sapiens protein
Buffer:	10 mM HEPES pH 8, 100 mM NaCl, 2.5 mM ATP, 2.5 mM MgCl2, and 0.1 mM Na3VO4, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 May 5

RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles Nature Communications 14(1) (2023)
Longo M, Roy S, Chen Y, Tomaszowski K, Arvai A, Pepper J, Boisvert R, Kunnimalaiyaan S, Keshvani C, Schild D, Bacolla A, Williams G, Tainer J, Schlacher K

R_g^Guinier	3.6	nm
D_max	14.0	nm
Volume^Porod	132	nm³

SASDR89 – Aromatic-L-amino-acid decarboxylase (AADC) - apo form

Aromatic-L-amino-acid decarboxylase (M17V) experimental SAS data

Sample:	Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer:	50 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Feb 28

Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: implication in AADC deficiency Protein Science (2023)
Bisello G, Ribeiro R, Perduca M, Belviso B, Polverino de' Laureto P, Giorgetti A, Caliandro R, Bertoldi M

R_g^Guinier	3.4	nm
D_max	15.8	nm
Volume^Porod	181	nm³

SASDR99 – Aromatic-L-amino-acid decarboxylase (AADC) - pyridoxal 5'-phosphate bound holo form

Sample:	Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer:	50 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Feb 28

R_g^Guinier	3.2	nm
D_max	14.9	nm
Volume^Porod	180	nm³

SASDRA9 – Aromatic-L-amino-acid decarboxylase (AADC) - dopa methylester DME-bound holo form

Sample:	Aromatic-L-amino-acid decarboxylase (M17V) dimer, 108 kDa Homo sapiens protein
Buffer:	50 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Feb 28

R_g^Guinier	3.0	nm
D_max	14.5	nm
Volume^Porod	154	nm³

SASDQR4 – Escherichia coli 6S RNA

6S RNA (SsrS gene) experimental SAS data

Sample:	6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Buffer:	20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Apr 4

Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Frontiers in Molecular Biosciences 10 (2023)
Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V

R_g^Guinier	6.1	nm
D_max	24.0	nm
Volume^Porod	175	nm³

SASDQS4 – Escherichia coli 6S:pRNA complex

6S RNA (SsrS gene)product RNA from E. coli 6S experimental SAS data

Escherichia coli 6S:pRNA complex Rg histogram

Sample:	6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA Product RNA from E. coli 6S monomer, 7 kDa Escherichia coli RNA
Buffer:	20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Apr 4

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	260	nm³

SASDRT5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ADP

Nitrogen fixation regulatory protein (Q409L) experimental SAS data

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J

R_g^Guinier	4.9	nm
D_max	17.9	nm
Volume^Porod	233	nm³

SASDRU5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ATP

Sample:	Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer:	50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5

R_g^Guinier	4.9	nm
D_max	18.0	nm
Volume^Porod	225	nm³

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