Small Angle Scattering Biological Data Bank SASBDB

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SASDP56 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J

R_g^Guinier

4.1

SASDP66 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	98	nm³

SASDP76 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	100	nm³

SASDP86 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	6.3	nm
D_max	24.0	nm

SASDP96 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	5.2	nm
D_max	18.0	nm

SASDPA6 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	4.8	nm
D_max	16.0	nm

SASDMX6 – 40-mer single stranded DNA inhibitor of DNA dC->dU-editing enzyme APOBEC3G

40-mer single stranded inhibitory DNA experimental SAS data

Sample:	40-mer single stranded inhibitory DNA monomer, 12 kDa DNA
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 8

Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G

R_g^Guinier	3.2	nm
D_max	11.8	nm
Volume^Porod	26	nm³

SASDMY6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length

DNA dC->dU-editing enzyme APOBEC-3G experimental SAS data

Sample:	DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	4.2	nm
D_max	13.3	nm
Volume^Porod	350	nm³

SASDMZ6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: tetramer

DNA dC->dU-editing enzyme APOBEC-3G40-mer single stranded inhibitory DNA experimental SAS data

Sample:	DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein 40-mer single stranded inhibitory DNA dimer, 24 kDa DNA
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	4.7	nm
D_max	16.2	nm
Volume^Porod	395	nm³

SASDM27 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: monomer

40-mer single stranded inhibitory DNADNA dC->dU-editing enzyme APOBEC-3G experimental SAS data

Sample:	40-mer single stranded inhibitory DNA monomer, 12 kDa DNA DNA dC->dU-editing enzyme APOBEC-3G monomer, 46 kDa Homo sapiens protein
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	118	nm³

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