Browse by DISSEMINATION: Published

SASDNH2 – Complex of Geobacter sulfurreducens pAgo with Sir2-like effector protein

Piwi domain proteinSir2 superfamily protein experimental SAS data
GASBOR model
Sample: Piwi domain protein monomer, 53 kDa Geobacter sulfurreducens (strain … protein
Sir2 superfamily protein monomer, 68 kDa Geobacter sulfurreducens (strain … protein
Buffer: 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 150 mM NaCl and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 27
Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion Nature Microbiology (2022)
Zaremba M, Dakineviciene D, Golovinas E, Zagorskaitė E, Stankunas E, Lopatina A, Sorek R, Manakova E, Ruksenaite A, Silanskas A, Asmontas S, Grybauskas A, Tylenyte U, Jurgelaitis E, Grigaitis R, Timinskas K, Venclovas Č, Siksnys V
RgGuinier 3.6 nm
Dmax 11.4 nm
VolumePorod 185 nm3

SASDQB5 – Elongation factor Tu from Mycobacterium tuberculosis

Elongation factor Tu experimental SAS data
DAMMIN model
Sample: Elongation factor Tu monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
Zhan B, Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 3.5 nm
Dmax 9.0 nm
VolumePorod 94 nm3

SASDQC5 – Elongation factor Ts from Mycobacterium tuberculosis

Elongation factor Ts experimental SAS data
DAMMIN model
Sample: Elongation factor Ts monomer, 29 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
Zhan B, Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 46 nm3

SASDQD5 – Elongation factor-Tu and Elongation factor-Ts complex from Mycobacterium tuberculosis

Elongation factor TuElongation factor Ts experimental SAS data
DAMMIN model
Sample: Elongation factor Tu monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
Elongation factor Ts monomer, 29 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
Zhan B, Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 111 nm3

SASDJS7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 7.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3

SASDNN3 – Peptidyl-prolyl cis-trans isomerase FKBP43, nucleoplasmin domain (amino acids 1-96)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 58 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 16
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 2.5 nm
Dmax 7.3 nm
VolumePorod 141 nm3

SASDNQ7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 3.6 nm
Dmax 12.4 nm
VolumePorod 164 nm3

SASDNR7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H2A/H2B histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43Histone H2A type 1-B/EHistone H2B type 1-C/E/F/G/I experimental SAS data
MULTIFOXS model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Histone H2A type 1-B/E monomer, 14 kDa Homo sapiens protein
Histone H2B type 1-C/E/F/G/I monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 194 nm3

SASDNS7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-136) -H3/H4 histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43Histone H3.1Histone H4 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein
Histone H3.1 dimer, 31 kDa Homo sapiens protein
Histone H4 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 4.4 nm
Dmax 14.1 nm
VolumePorod 226 nm3