Browse by DISSEMINATION: Published

SASDNT7 – Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-164)

Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 96 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 28
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 180 nm3

SASDPD3 – Second exon splicing silencer 2p (ESS2p) RNA, apo

The second exon splicing silencer 2p experimental SAS data
AMBER model
Sample: The second exon splicing silencer 2p monomer, 14 kDa RNA
Buffer: 20 mM Bis-Tris, 20 mM NaCl, pH: 6.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 1
Encoded Conformational Dynamics of the HIV Splice Site A3 Regulatory Locus: Implications for Differential Binding of hnRNP Splicing Auxiliary Factors. J Mol Biol 434(18):167728 (2022)
Chiu LY, Emery A, Jain N, Sugarman A, Kendrick N, Luo L, Ford W, Swanstrom R, Tolbert BS
RgGuinier 2.1 nm
Dmax 6.9 nm
VolumePorod 21 nm3

SASDPK4 – His-tagged RdfS Excisionase monomeric in solution

DNA-binding protein experimental SAS data
His-tagged RdfS Excisionase monomeric in solution Rg histogram
Sample: DNA-binding protein monomer, 13 kDa Mesorhizobium loti protein
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element Acta Crystallographica Section D Structural Biology 78(10) (2022)
Verdonk C, Marshall A, Ramsay J, Bond C
RgGuinier 2.2 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDP79 – Chromatin assembly factor 1 - long alpha-helix domain

Chromatin assembly factor 1 subunit A experimental SAS data
DAMMIN model
Sample: Chromatin assembly factor 1 subunit A monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.1 mM TCEP, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2021 Oct 28
Unorthodox PCNA Binding by Chromatin Assembly Factor 1 International Journal of Molecular Sciences 23(19):11099 (2022)
Gopinathan Nair A, Rabas N, Lejon S, Homiski C, Osborne M, Cyr N, Sverzhinsky A, Melendy T, Pascal J, Laue E, Borden K, Omichinski J, Verreault A
RgGuinier 4.1 nm
Dmax 17.2 nm
VolumePorod 54 nm3

SASDNM3 – Sperm-expressed surface protein

Sperm acrosome membrane-associated protein 6 experimental SAS data
DAMFILT model
Sample: Sperm acrosome membrane-associated protein 6 monomer, 25 kDa Homo sapiens protein
Buffer: 2.7 mM KCl, 137 mM NaCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Dec 11
SPACA6 ectodomain structure reveals a conserved superfamily of gamete fusion-associated proteins. Commun Biol 5(1):984 (2022)
Vance TDR, Yip P, Jiménez E, Li S, Gawol D, Byrnes J, Usón I, Ziyyat A, Lee JE
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 34 nm3

SASDPT3 – Histone-lysine N-methyltransferase multi protein complex: MLL1-WDR5-RBBP5-ASH2L

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Retinoblastoma-binding protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2 experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. iScience 25(9):104948 (2022)
Zhao L, Huang N, Mencius J, Li Y, Xu Y, Zheng Y, He W, Li N, Zheng J, Zhuang M, Quan S, Chen Y
RgGuinier 5.2 nm
Dmax 18.5 nm
VolumePorod 386 nm3

SASDPU3 – Histone-lysine N-methyltransferase multi protein complex bound to protein dpy-30 homologue: MLL1-WDR5-RBBP5-ASH2L-DPY30

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Retinoblastoma-binding protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Protein dpy-30 homolog experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Protein dpy-30 homolog dimer, 23 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. iScience 25(9):104948 (2022)
Zhao L, Huang N, Mencius J, Li Y, Xu Y, Zheng Y, He W, Li N, Zheng J, Zhuang M, Quan S, Chen Y
RgGuinier 5.4 nm
Dmax 17.5 nm
VolumePorod 411 nm3

SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence

Transposon Tn3 resolvaseTn3 res, resolvase binding site II experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein
Tn3 res, resolvase binding site II monomer, 32 kDa DNA
Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6
Structural basis for topological regulation of Tn3 resolvase. Nucleic Acids Res (2022)
Montaño SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
RgGuinier 5.0 nm
Dmax 14.9 nm
VolumePorod 217 nm3

SASDPD4 – Ca2+-calmodulin

Calmodulin-1 experimental SAS data
Calmodulin-1 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 2.1 nm
Dmax 6.9 nm
VolumePorod 23 nm3

SASDPE4 – P2X7 ballast domain

P2X purinoceptor 7 experimental SAS data
P2X purinoceptor 7 Kratky plot
Sample: P2X purinoceptor 7 trimer, 74 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 94 nm3