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SASDTB7 – RNase P RNA in the presence of 1.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.2 nm
Dmax 15.6 nm
VolumePorod 370 nm3

SASDTC7 – RNase P RNA in the presence of 1.5 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,1.5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.0 nm
Dmax 16.2 nm
VolumePorod 352 nm3

SASDTD7 – RNase P RNA in the presence of 2.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 2.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.0 nm
Dmax 15.7 nm
VolumePorod 316 nm3

SASDTE7 – RNase P RNA in the presence of 2.5 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 2.5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 306 nm3

SASDTF7 – RNase P RNA in the presence of 3.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 3 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 301 nm3

SASDTG7 – RNase P RNA in the presence of 5.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.6 nm
VolumePorod 277 nm3

SASDTH7 – RNase P RNA in the presence of 8.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 8.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDTJ7 – RNase P RNA in the presence of 10.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,10.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.8 nm
Dmax 14.6 nm
VolumePorod 285 nm3

SASDQJ7 – Alpha Synuclein at 283 K and concentration of 8 mg/ml

Human alpha-synuclein experimental SAS data
ALPHAFOLD model
Sample: Human alpha-synuclein monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2015 Mar 18
Visualizing gaussian-chain like structural models of human α-synuclein in monomeric pre-fibrillar state: Solution SAXS data and modeling analysis. Int J Biol Macromol 288:138614 (2024)
Dey M, Gupta A, Badmalia MD, Ashish, Sharma D
RgGuinier 4.7 nm
Dmax 17.6 nm
VolumePorod 81 nm3

SASDQK7 – Alpha Synuclein at 283 K and concentration of 3.8 mg/ml

Human alpha-synuclein experimental SAS data
Human alpha-synuclein Kratky plot
Sample: Human alpha-synuclein monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2015 Mar 18
Visualizing gaussian-chain like structural models of human α-synuclein in monomeric pre-fibrillar state: Solution SAXS data and modeling analysis. Int J Biol Macromol 288:138614 (2024)
Dey M, Gupta A, Badmalia MD, Ashish, Sharma D
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 76 nm3